The role of selenium added to pulmonary preservation solutions in isolated guinea pig lungs

• Published in: The Journal of thoracic and cardiovascular surgery Vol. 108; no. 5; pp. 922 - 927
• Main Authors: Soncul, Halim, Kaptanoglu, Melih, Oz, Eser, Halit, Velit, Bilgehan, Ayse, Cayci, Banu, Gokgoz, Levent, Turkozan, Nurten, Ersoz, Ali
• Format: Journal Article
• Language: English
• Published: United States AATS/WTSA 01.11.1994
• Subjects: Adenosine Deaminase - analysis; Animals; Guinea Pigs; Humans; Ischemia - metabolism; Ischemia - pathology; Lung - blood supply; Lung - drug effects; Male; Malondialdehyde - analysis; Organ Preservation - methods; Pulmonary Alveoli - pathology; Pulmonary Artery - physiology; Reperfusion Injury - metabolism; Reperfusion Injury - prevention & control; Selenium - pharmacology
• ISSN: 0022-5223; 1097-685X
• DOI: 10.1016/S0022-5223(94)70192-X

An experimental comparative study on isolated guinea pig lungs has been undertaken to determine the probable beneficial effects of adding selenium to pulmonary preservation solutions in lung ischemia. The isolated lungs (n = 10 in each group) previously being perfused by oxygenated Krebs-Henseleit solution were put in normothermic ischemic conditions just after the infusion of 30 ml of pulmonary preservation solution (Euro-Collins in the control group, Euro-Collins plus selenium 10(-3) mol in the experiment group). After 3 hours of normothermic ischemia the lungs were reperfused with the same buffer for 20 minutes. Pulmonary artery pressures, tissue malondialdehyde levels, and adenosine deaminase levels of the perfusate were measured before and after the ischemic period and also at the end of reperfusion. An electron microscopic analysis was performed on the lung tissues at the end of the experimental procedure. According to our data, the addition of selenium to pulmonary preservation solution showed a significant protective effect regarding both ischemic and reperfusion injury.