Virus particle detection by solid phase immunocapture and atomic force microscopy

• Published in: Biochemical and biophysical research communications Vol. 311; no. 2; pp. 540 - 545
• Main Authors: Nettikadan, Saju R, Johnson, James C, Mosher, Curtis, Henderson, Eric
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 14.11.2003
• Subjects: Atomic force microscope; atomic force microscopy; Canine parvovirus; Coxsackievirus; Equipment Design; Equipment Failure Analysis; Immunoassay - instrumentation; Immunoassay - methods; Microscopy, Atomic Force - instrumentation; Microscopy, Atomic Force - methods; Phage fd; Protein Array Analysis - instrumentation; Protein Array Analysis - methods; Reproducibility of Results; Sensitivity and Specificity; Solid-phase immunocapture; ViriChip; Virus detection; Viruses - isolation & purification; Virus detection; ViriChip; Atomic force microscope; Solid-phase immunocapture
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2003.10.022

A novel application of atomic force microscopy (AFM) in the rapid, label-free detection and identification of viruses is described. Multiplexed, miniaturized antibody domains were constructed using “ink-jet” protein arraying technology. The solid-phase affinity substrate termed the “ViriChip” was used in the immunocapture of bacteriophage fd, canine parvoviruses, and coxsackieviruses and analyzed by AFM. Immunocapture was found to be antibody-specific with a sensitivity of 10 8 pfu/ml in 30 min. Virus binding was found to be linear for concentration between 10 8 and 10 10 pfu/ml and did not reach saturation through 4 h.