Protection of hamsters from amebic liver abscess formation by a monoclonal antibody to a 150-kDa surface lectin of Entamoeba histolytica

• Published in: Parasitology research (1987) Vol. 85; no. 1; pp. 78 - 80
• Main Authors: CHENG, X.-J, TACHIBANA, H, KANEDA, Y
• Format: Journal Article
• Language: English
• Published: Berlin Springer 1999
• Subjects: Animals; Antibodies, Monoclonal - therapeutic use; Antigens, Protozoan - immunology; Biological and medical sciences; Cricetinae; Entamoeba histolytica - immunology; Experimental protozoal diseases and models; Infectious diseases; Lectins - immunology; Liver Abscess, Amebic - immunology; Liver Abscess, Amebic - prevention & control; Male; Medical sciences; Parasitic diseases; Protozoal diseases; Protozoan Vaccines; Protozoa; Host parasite relation; Lectin; Animal model; Protozoal disease; Lobosea; Liver; Rodentia; Abscess; Parasite; Entamoeba histolytica; Host; Parasitosis; Monoclonal antibody; Cell surface; Infection; Prevention; Vertebrata; Mammalia; Amebiasis; Formation; Passive immunization; Humoral immunity; Hamster
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s004360050510

We examined the effects of passive immunization with a monoclonal antibody (EH3015) that recognizes a 150-kDa surface lectin of Entamoeba histolytica on amebic liver-abscess formation in hamsters. The hamsters were inoculated i.p with 0.1, 1.0, or 10 mg of EH3015 at 24 h prior to an intrahepatic challenge with 10(5) trophozoites of E. histolytica. In hamsters treated with 1.0 and 10 mg of EH3015 the incidence of liver abscesses was significantly reduced. These results demonstrate that monoclonal antibody EH3015 can prevent the development of amebic liver abscesses and that the 150-kDa lectin may be a protective antigen on the surface of E. histolytica.