Protection and antibody responses in different strains of mouse immunized with plasmid DNAs encoding influenza virus haemagglutinin, neuraminidase and nucleoprotein

• Published in: Journal of general virology Vol. 80; no. 10; pp. 2559 - 2564
• Main Authors: Chen, Ze, Yoshikawa, Tomoki, Kadowaki, Shin-etsu, Hagiwara, Yukari, Matsuo, Kazutoshi, Asanuma, Hideki, Aizawa, Chikara, Kurata, Takeshi, Tamura, Shin-ichi
• Format: Journal Article
• Language: English
• Published: England Soc General Microbiol 01.10.1999
• Subjects: Animals; Antibodies, Viral - immunology; DNA, Viral - immunology; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Humans; Influenza A virus - genetics; Influenza A virus - immunology; Influenza Vaccines - genetics; Influenza Vaccines - immunology; influenza virus; Influenza, Human - prevention & control; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Neuraminidase - genetics; Neuraminidase - immunology; Nucleoproteins - genetics; Nucleoproteins - immunology; Plasmids - immunology; RNA-Binding Proteins; Vaccination; Vaccines, DNA - genetics; Vaccines, DNA - immunology; Viral Core Proteins - genetics; Viral Core Proteins - immunology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-80-10-2559

Department of Pathology, National Institute of Infectious Diseases, Toyama 1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan 1 Research Center for Biologicals, Kitasato Institute, 6-111 Arai, Kitamoto-shi, Saitama 364-0026, Japan 2 Author for correspondence: Shin-ichi Tamura.Fax +81 3 5285 1189. e-mail stamura{at}nih.go.jp Protection against influenza virus infection and antibody responses in mice vaccinated with plasmid DNAs encoding haemagglutinin (HA), neuraminidase (NA) and nucleoprotein (NP) were compared among BALB/c (H-2 d ), B10 (H-2 b ) and C3H (H-2 k ) mice. Mice were inoculated with each DNA construct twice, 3 weeks apart, at a dose of 1 µg per mouse by particle-mediated DNA transfer (gene gun) to the epidermis. They were challenged with a lethal dose of the homologous virus 7 days after the second vaccination. NA-DNA provided significant protection in all strains of mouse, whereas HA-DNA afforded significant protection only in BALB/c mice. The serum antibody titres against NA or HA molecules in BALB/c, C3H and B10 mice were high, intermediate and low, respectively. NP-DNA failed to provide protection in any strain of mouse, and elicited low titres of anti-NP antibodies. These results suggest that NA-DNA can be used as a vaccine component to provide effective protection against influenza virus infection in various strains of mouse.