A new dispensable genetic locus of the terminus region involved in control of cell division in Escherichia coli

Temperature-sensitive mutants defective in cell division were isolated after localised mutagenesis of the terminus region of the Escherichia coli chromosome. The defective gene in one of these mutants, dicA, was mapped at 34.9 min by linkage with manA and with three physically characterized Tn10 ins...

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Bibliographic Details
Published inMolecular & general genetics Vol. 201; no. 2; p. 146
Main Authors Béjar, S, Bouché, J P
Format Journal Article
LanguageEnglish
Published Germany 01.01.1985
Subjects
Escherichia coli - genetics
Escherichia coli - growth & development
Genes, Bacterial
Genetic Vectors
Genotype
Mutation
Plasmids
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Summary:Temperature-sensitive mutants defective in cell division were isolated after localised mutagenesis of the terminus region of the Escherichia coli chromosome. The defective gene in one of these mutants, dicA, was mapped at 34.9 min by linkage with manA and with three physically characterized Tn10 insertions. Temperature-sensitivity conferred by mutation dicA1 in a recA background [corrected] was suppressed by the presence of hybrid plasmids carrying the wild-type gene. In addition, the mutation was suppressed either by tranposon inactivation of a nearby gene, dicB, or by deletion of the entire dicA-dicB interval. These results define the dicA-dicB locus as a new dispensable genetic cluster involved in the control of cell division.
ISSN:0026-8925
DOI:10.1007/BF00425651