Myceligenerans xiligouense gen. nov., sp. nov., a novel hyphae-forming member of the family Promicromonosporaceae

• Published in: International journal of systematic and evolutionary microbiology Vol. 54; no. 4; pp. 1287 - 1293
• Main Authors: Cui, Xiaolong, Schumann, Peter, Stackebrandt, Erko, Kroppenstedt, Reiner M, Pukall, Rudiger, Xu, Lihua, Rohde, Manfred, Jiang, Chenglin
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.07.2004; Society for General Microbiology
• Subjects: Actinomycetales - classification; Actinomycetales - cytology; Actinomycetales - isolation & purification; Actinomycetales - physiology; Bacterial Typing Techniques; Bacteriology; Base Composition; Biological and medical sciences; China; DNA, Bacterial - chemistry; DNA, Ribosomal - chemistry; Fundamental and applied biological sciences. Psychology; Genes, rRNA; Hexoses - analysis; Microbiology; Microscopy, Electron, Scanning; Molecular Sequence Data; Myceligenerans xiligouense; Mycelium - cytology; Peptidoglycan - chemistry; Phospholipids - analysis; Phylogeny; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Sequence Homology; Soil Microbiology; Spores, Bacterial - cytology; Systematics; Vitamin K 2 - analysis; China; Taxonomy; New genus; New species
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.03046-0

1 The Key Laboratory for Microbial Resources of Ministry of Education, Yunnan Institute of Microbiology, Yunnan University, Kunming, Yunnan 650091, P. R. China 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen, Mascheroder Weg 1b, D-38124 Braunschweig, Germany 3 GBF – Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, D-38124 Braunschweig, Germany Correspondence Erko Stackebrandt erko{at}dsmz.de Strain XLG9A10.2 T was isolated from an alkaline salt marsh soil in western China. 16S rRNA gene sequence analysis indicated that strain XLG9A10.2 T constitutes a distinct lineage within the family Promicromonosporaceae , sharing 94·8–95·1 % gene similarity with members of the genus Promicromonospora and 94·4–95·7 % similarity with those of Xylanimonas and related genera. The general colony and cell morphology of strain XLG9A10.2 T is similar to that of members of Promicromonospora , but differs from members of the genus Xylanimonas in forming a well-developed branching mycelium and production of coccoid spores. Strain XLG9A10.2 T shows the peptidoglycan type A4 ( L -lys L -thr D -Glu), contains glucose, mannose and galactose as whole cell sugars and has MK-9(H 4 ) and MK-9(H 6 ) as major menaquinones, while phospholipids are phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids and one unidentified glycolipid. The DNA base composition is 71·9 mol% G+C. On the basis of morphological, chemotaxonomic, metabolic and phylogenetic differences from other species of Promicromonosporaceae , a new genus and species, Myceligenerans xiligouense gen. nov., sp. nov., is proposed. The type strain is XLG9A10.2 T (=DSM 15700 T =CGMCC 1.3458 T .) The GenBank accession number for the 16S rRNA gene sequence of strain XLG9A10.2 T is AY354285 . Additional images showing the morphology of M. xiligouense are available in IJSEM Online.