A new spiroplasma isolate from the field cricket (Gryllus bimaculatus) in Taiwan

• Published in: Journal of invertebrate pathology Vol. 120; pp. 4 - 8
• Main Authors: Nai, Yu-Shin, Su, Ping-Yi, Hsu, Yu-Hsiang, Chiang, Ching-Hao, Kim, Jae Su, Chen, Yue-Wen, Wang, Chung-Hsiung
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2014; Elsevier
• Subjects: 16S rRNA; Animals; Biological and medical sciences; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gryllidae - microbiology; Gryllus bimaculatus; Insecta; Invertebrates; Pathology; Phylogeny; Reverse Transcriptase Polymerase Chain Reaction; Spiroplasma; Spiroplasma - genetics; Taiwan; Asia; Taiwan; Far east; 16S rRNA; Spiroplasma; Phylogeny; Gryllus bimaculatus; Subtropical zone; Taxonomy; Insecta; Orthoptera; Gryllidae; Mollicutes; Mycoplasmatales; Spiroplasmataceae; Arthropoda; 16S-RNA; Bacteria; Invertebrata
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2014.04.006

[Display omitted] •A spiroplasma isolate was recovered from, Gryllus bimaculatus in northern Taiwan.•This isolate is closely related to Spiroplasma platyhelix from the dragonfly.•Based on the phylogenetic analysis, this isolate clustered in the Ixodetis clade.•We suggest that this spiroplasma isolate is a new member of the genus Spiroplasma. We briefly described the morphology and transmission pathway of a Spiroplasma sp. isolated from the field cricket, Gryllus bimaculatus in Taiwan, followed by the phylogenetic analysis based on the 16S rRNA gene sequence. The cricket spiroplasma infected the hemolymph, gut, muscle tissues and tracheal cells; therefore we suggest that the pathogen invaded tissues and organs from the hemolymph through the tracheal system and the endoplasmic reticular system. Based on 16S rRNA gene sequences and the phylogeny, this spiroplasma was most closely related to Spiroplasma platyhelix (Identity=95%) isolated from the dragonfly Pachydiplax longipennis and belongs to the Ixodetis clade.