Establishment of matrix metalloproteinase 3 time-resolved immunoassay and some potential clinical applications

• Published in: Analytical biochemistry Vol. 666; p. 115072
• Main Authors: Fu, Yulin, Wang, Xiaoyan, Chen, Xindong, Hong, Jianfeng, Qin, Yuan, Zhou, Zixuan, Zhou, Xiumei, Wang, Yigang, Zhou, Jingnan, Fang, Hongming, Liu, Pengfei, Huang, Biao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.04.2023
• Subjects: Antibodies; blood serum; Colorectal cancer; colorectal neoplasms; Diagnosis; Double antibody sandwich; fluorescent antibody technique; Fluoroimmunoassay - methods; Humans; immunoassays; Matrix Metalloproteinase 3; metastasis; MMP-3; Serum; stromelysin 1; TRFIA; trypsinogen; Diagnosis; Double antibody sandwich; TRFIA; MMP-3; Colorectal cancer
• ISSN: 0003-2697; 1096-0309; 1096-0309
• DOI: 10.1016/j.ab.2023.115072

To develop a highly sensitive time-resolved fluorescence immunoassay (TRFIA) for the detection of serum matrix metalloproteinase-3 (MMP-3) and to assess MMP-3's clinical value in patients with colorectal cancer (CRC).st. MMP-3 levels were established using the double antibody sandwich technique. The MMP-3 TRFIA technique was developed and optimized, and its linearity, sensitivity, accuracy, specificity, and recovery were assessed. Then, serum concentrations in healthy individuals and patients with CRC were determined by MMP-3 TRFIA. The linear range of MMP-3 TRFIA was 0.73–500 ng/mL. MMP-3 TRFIA had an intra-batch precision range of 2.16%–7.10% percent and an inter-batch precision range of 3.99%–11.21%. MMP-3, tumor-associated trypsinogen 2, and AFP had no cross reaction.The recovery is between 90% and 110%, and had no serum interference. Patients with CRC had serum MMP-3 levels (73.95 ± 78.43 ng/mL) that were considerably higher than those of healthy individuals (21.45 ± 11.12 ng/mL), and those with metastasis had serum MMP-3 levels (95.89 ± 76.21 ng/mL) that were considerably higher than those of patients without metastasis (52.74 ± 47.25 ng/mL). A highly sensitive MMP-3 TRFIA assay was successfully developed, and serum MMP-3 may be associated with CRC invasion and metastasis. Therefore, MMP-3 can be used in the auxiliary diagnosis of CRC. •A time-resolved fluorescence immunoassay method for the detection of serum MMP-3 concentration was successfully established.•We found the levels of serum MMP-3 were higher in patients with colorectal cancer than healthy subjects.•Detection of serum MMP-3 concentration can distinguish patients with and without metastatic colorectal cancer.•MMP-3 can be used as an auxiliary detection marker for colorectal cancer.