Human erythrocyte membrane fluidity and insulin binding are independent of dietary trans fatty acids

• Published in: The Journal of nutritional biochemistry Vol. 5; no. 12; pp. 591 - 598
• Main Authors: Berlin, Elliott, Bhathena, Sam J., Judd, Joseph T., Clevidence, Beverly A., Peters, Renee C.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.1994; Elsevier Science
• Subjects: ACIDE GRAS; ACIDE GRAS SATURE; ACIDE LINOLEIQUE; ACIDE OLEIQUE; ACIDE PROPIONIQUE; ACIDO LINOLEICO; ACIDO OLEICO; ACIDO PROPIONICO; ACIDOS GRASOS; ACIDOS GRASOS SATURADOS; binding proteins; Biological and medical sciences; CHOLESTEROL; COLESTEROL; CONCEPTION; CORPS GRAS; DIETA; dietary fat; DISENO; ERITROCITOS; ERYTHROCYTE; erythrocytes; EXPERIMENTACION; experimental design; EXPERIMENTATION; Feeding. Feeding behavior; FEMME; FLUORESCENCE; FLUORESCENCIA; fluorescent probes; FOSFOLIPIDOS; Fundamental and applied biological sciences. Psychology; GRASAS; HOMBRES; HOMME; hormone receptors; INSTRUMENT DE MESURE; INSTRUMENTOS DE MEDICION; insulin; insulin binding; insulin receptor; INSULINA; INSULINE; linoleic acid; MEMBRANAS CELULARES; MEMBRANE CELLULAIRE; membrane fluidity; men; MOUVEMENT; MOVIMIENTO; MUJERES; oleic acid; PHOSPHATIDE; phospholipids; probes (equipment); propionic acid; PROTEINAS AGLUTINANTES; PROTEINE DE LIAISON; RECEPTEUR D'HORMONE; RECEPTORES DE HORMONAS; REGIME ALIMENTAIRE; saturated fatty acids; TEMPERATURA; TEMPERATURE; trans fatty acids; Vertebrates: anatomy and physiology, studies on body, several organs or systems; women; trans fatty acids; insulin binding; insulin receptor; fluorescent probes; membrane fluidity; Human; Pancreatic hormone; Red blood cell; Lipids; Insulin; Feeding; Blood cell; Fluidity; Diet; Trans stereoisomer; Plasma membrane; Unsaturated fatty acid; Hormonal receptor
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/0955-2863(94)90014-0

Substitution of selected saturated fatty acids of the diet of 29 men and 29 women with cis or trans monounsaturated fatty acids did not affect erythrocyte membrane fluidity, insulin binding, and the membrane cholesterol and phospholipid concentrations. Subjects were fed four different controlled diets with a total fatty acid content of 39 to 40 energy percent for four 6-week periods in a Latin square design. The diets were: (1) high oleic acid (16.7 energy percent oleic); (2) moderate trans (3.8 energy percent trans fatty acids); (3) high trans (6.6 energy percent trans fatty acids); and saturated (16.2 energy percent lauric + myristic + palmitic acids). There were no significant diet effects on red cell ghost fluidity determined by fluorescence polarization of the hydrocarbon probe 1,6-diphenyl-1,3,5-hexatriene (DPH) and the polar analog trimethylammonium-DPH (TMA-DPH). There were limited diet effects on fluidity of membranes as determined with DPH-propionic acid (DPH-PA) for the men. Insulin binding was more closely associated with anisotropy of fluorescence of the surface probe, DPH-PA, than with that of the other probes, which is compatible with the localization of the insulin receptor in a domain at the cell membrane surface.