Long interspersed element-1 open reading frame 1 protein expression profiles in ovarian cancers

• Published in: Genes & genomics Vol. 39; no. 10; pp. 1157 - 1162
• Main Authors: Yun, Ka Yeong, Ko, Eun Ji, Kim, Heung Yeol, Lee, Ji Young, Eo, Wan Kyu, Ock, Mee Sun, Kim, Heui-Soo, Kim, Ki Hyung, Cha, Hee-Jae
• Format: Journal Article
• Language: English
• Published: Seoul The Genetics Society of Korea 01.10.2017; Springer Nature B.V; 한국유전학회
• Subjects: Animal Genetics and Genomics; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; DNA microarrays; fluorescent antibody technique; genome; Genomes; Human Genetics; humans; image analysis; Image processing; Immunofluorescence; Life Sciences; Long interspersed nucleotide elements; Lymph nodes; Medical prognosis; Metastases; Metastasis; mice; microarray technology; Microbial Genetics and Genomics; monoclonal antibodies; open reading frames; Ovarian cancer; ovarian neoplasms; patients; Plant Genetics and Genomics; prognosis; protein synthesis; proteins; Research Article; retrotransposons; tissues; 생물학; LINE-1; Protein expression; ORFp1; Ovarian cancer
• ISSN: 1976-9571; 2092-9293
• DOI: 10.1007/s13258-017-0589-5

Long interspersed element-1 (LINE-1) retrotransposons are autonomous mobile DNA elements with unique activity that account for about one-fifth of the human genome. Recently, it has been reported that the expression of LINE-1 is closely related to cancer prognosis, and LINE-1 hypomethylation might contribute to the acquisition of aggressive tumor behavior. Despite the importance of LINE-1 expression in cancers, research on the expression of LINE-1 open reading frame (ORF) proteins is very limited. Here, we investigated the expression profiles of LINE-1 ORF1p in ovarian cancer tissue microarrays containing 100 surgical specimens including adjacent normal ovary tissue, primary ovarian cancers, and metastatic ovarian cancers in lymph node. The tissue microarray was stained with mouse monoclonal antibody to LINE-1 ORFp1 for immunofluorescence analysis, and expression levels were evaluated by image analysis. LINE-1 ORFp was significantly overexpressed in ovarian cancers compared with normal tissues and especially upregulated in metastatic ovarian cancers. In addition, the expression of LINE-1 ORF1p was significantly higher in older ovarian cancer patients compared with young patients. These results indicate that expression of LINE-1 ORF1p is related to the progression of ovarian cancers and, in particular, to the age of the patient and the metastatic potential of the cancer.