Ligularia fischeri Extract Protects Against Oxidative-Stress-Induced Neurotoxicity in Mice and PC12 Cells

• Published in: Journal of medicinal food Vol. 17; no. 11; pp. 1222 - 1231
• Main Authors: Choi, Soo Jung, Kim, Jae Kyeom, Suh, Soo Hwan, Kim, Cho Rong, Kim, Hye Kyung, Kim, Chang-Ju, Park, Gwi Gun, Park, Cheung-Seog, Shin, Dong-Hoon
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.11.2014
• Subjects: active ingredients; Alzheimer disease; Alzheimer Disease - metabolism; Alzheimer Disease - prevention & control; amyloid; Amyloid beta-Peptides - metabolism; Amyloid beta-Peptides - toxicity; Animals; antioxidant activity; Antioxidants - pharmacology; Antioxidants - therapeutic use; Asteraceae - chemistry; Avoidance Learning - drug effects; brain; Brain - drug effects; chlorogenic acid; Chlorogenic Acid - pharmacology; Chlorogenic Acid - therapeutic use; Cognition Disorders - chemically induced; Cognition Disorders - genetics; Cognition Disorders - prevention & control; diet; DNA fragmentation; DNA Fragmentation - drug effects; high performance liquid chromatography; Hydrogen Peroxide - metabolism; Hydrogen Peroxide - toxicity; Learning Disorders - chemically induced; Learning Disorders - prevention & control; Ligularia fischeri; lipid peroxidation; Lipid Peroxidation - drug effects; Male; memory; Memory - drug effects; Memory Disorders - chemically induced; Memory Disorders - prevention & control; mice; Mice, Inbred ICR; Neuroprotective Agents - pharmacology; Neuroprotective Agents - therapeutic use; neurotoxicity; Neurotoxicity Syndromes - genetics; Neurotoxicity Syndromes - prevention & control; oxidative stress; Oxidative Stress - drug effects; PC12 Cells; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; protective effect; Rats; amyloid beta peptide; 3-O-caffeoylquinic acid; Ligularia fischeri; oxidative stress
• ISSN: 1557-7600; 1557-7600
• DOI: 10.1089/jmf.2013.3014

Alzheimer's disease (AD) is pathologically characterized by the presence of amyloid plaques in brain and the overproduction of amyloid beta (Aβ), leading to learning and memory impairment and intense oxidative stress. In this study, the protective effect of Ligularia fischeri extract was investigated using PC12 cells. L. fischeri extract attenuated hydrogen-peroxide-induced DNA fragmentation in cells. In vivo behavioral tests were performed to examine the effects of the extract on amyloid-β peptide₁₋₄₂-induced impairment of learning and memory in mice. A diet containing the extract increased alternation behaviors in the Y-maze test and step-through latency of passive avoidance task. Moreover, we found that consumption of the extract decreased lipid peroxidation in a biochemical study of brain tissue in mice. High-performance liquid chromatography was used to identify the active compounds in the extract. These results suggest that L. fischeri extract could be protective against Aβ-induced neurotoxicity, possibly due to the antioxidative capacity of its constituent, 3-O-caffeoylquinic acid.