Note: Raman microspectroscopy integrated with fluorescence and dark field imaging

A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling...

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Bibliographic Details
Published inReview of scientific instruments Vol. 85; no. 5; p. 056109
Main Authors Li, Haibo, Wang, Hailong, Huang, Dianshuai, Liang, Lijia, Gu, Yuejiao, Liang, Chongyang, Xu, Shuping, Xu, Weiqing
Format Journal Article
LanguageEnglish
Published United States 01.05.2014
Subjects
Cell Line, Tumor
Fluorescence
Humans
Optical Imaging - instrumentation
Optical Imaging - methods
Spectrum Analysis, Raman - instrumentation
Spectrum Analysis, Raman - methods
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Summary:A Raman detection platform integrated with both fluorescence and dark field microscopes was built for in situ Raman detection with the assistance of fluorescence and dark field imaging to locate the target micro regions. Cells and organelles can be easily found via fluorescence imaging with labeling techniques. Besides, nano-sized particles could be observed and located by dark field microscopes. Therefore, comparing with the commercial Raman spectrometers, much more researches based on Raman spectroscopy could be carried out on this integrated Raman platform, especially in the fields of analyzing biological tissues and subwavelength samples.
ISSN:1089-7623
DOI:10.1063/1.4878715