Lipid metabolism related gene-expression profiling in liver, skeletal muscle and adipose tissue in crossbred Duroc and Pietrain Pigs

• Published in: Comparative biochemistry and physiology. Part D, Genomics & proteomics Vol. 2; no. 3; pp. 200 - 206
• Main Authors: Reiter, Sara S., Halsey, Charles H.C., Stronach, Benjamin M., Bartosh, Julia L., Owsley, W. Frank, Bergen, Werner G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.09.2007
• Subjects: Adipose; Domestic pigs; Gene expression; Genetic background; Lipid metabolism; Liver; Skeletal muscle; Adipose; Genetic background; Liver; Lipid metabolism; Gene expression; Skeletal muscle; Domestic pigs
• ISSN: 1744-117X; 1878-0407
• DOI: 10.1016/j.cbd.2007.04.008

Body-weight differences in animals may be ascribed to genetic and environmental factors. Here we utilized two divergent porcine genotypes, the highly muscled, leaner Pietrian × Yorkshire pigs and less muscled, fatter Duroc × Yorkshire growing pigs (75–110 kg), to examine the role of genetic background on expression of genes associated with anabolic (Fatty acid synthase, FAS; glucose transporter 4, GLUT-4; stearoyl CoA desaturase, SCD; Sterol regulatory binding protein-1, SREBP-1; leptin) and catabolic lipid metabolism (Carnitine palmitoyltransferase-1B, CPT-1B; acyl-CoA dehydrogenase, ACDH) in adipose tissue (AT), liver (L) and skeletal muscle (SKM). Pietrain pigs had lower mRNA abundance for FAS, SREBP-1, SCD and leptin in AT and L, but higher mRNA abundance for L ACDH and SKM ACDH and CPT-1B than Durocs. Duroc pigs exhibited higher expression of FAS, SREBP-1, SCD, leptin in AT and FAS in L and lower expression of ACDH and CPT-1B in L SKM. GLUT-4 expression did not differ in SKM between the two genotypes. Feeding of a beta adrenergic agonist (Paylean) for 52 days lowered expression of lipid anabolic and enhanced lipid catabolic genes expressions similarly in both genotypes. Overall, the lipid metabolism genes differential expression patterns documented here showed that in Pietrain pigs mRNA abundances of synthesis genes were lower and of catabolic genes were higher than in Duroc pigs.