Site-selective modification of peptide backbones

• Published in: Organic chemistry frontiers an international journal of organic chemistry Vol. 8; no. 23; pp. 6720 - 6759
• Main Authors: Boto, Alicia, González, Concepción C, Hernández, Dácil, Romero-Estudillo, Iván, Saavedra, Carlos J
• Format: Journal Article
• Language: English
• Published: London Royal Society of Chemistry 23.11.2021
• Subjects: Backbone; Biological properties; Biopolymers; Chemical reactions; Conformation; Enzymes; Hydrogen bonding; Hydrogen bonds; Organic chemistry; Peptides; Rigidity; Selectivity; Stereoselectivity
• ISSN: 2052-4110; 2052-4110
• DOI: 10.1039/d1qo00892g

The site-selective modification of peptide backbones allows an outstanding fine-tuning of peptide conformation, folding ability, and physico-chemical and biological properties. However, to achieve selectivity in the core of these biopolymers is challenging. In the last few years, many advances towards this goal have been developed. This review addresses the selective modification of Cα- and N-positions, from the use of “customizable units” to the residue-directed introduction of substituents. The site-selective modification of the peptide bond, i.e. the formation of thioamides or heterocycles, which alters backbone rigidity and ability to form hydrogen bonds or recognition by enzymes, is also described. Moreover, not only the modifications in internal backbone positions, but also in the N- and C-termini are discussed. In addition to chemical methodologies, the review addresses some reactions, catalyzed by natural or engineered enzymes, that afford unprecedent regio- and stereoselectivity in backbone modifications.