Plasma hemoglobin determination by recording derivative spectrophotometry

Recording derivative spectrophotometry (DS) is a technic for analyzing absorbance curves by measuring the derivative (slope) of those curves. The derivative is a mathematical function that enhances rapid changes of direction of a curve and suppresses slow changes. We are presenting a simple, rapid,...

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Bibliographic Details
Published inAmerican journal of clinical pathology Vol. 85; no. 3; pp. 342 - 347
Main Authors SOLONI, F. G, CUNNINGHAM, M. T, AMAZON, K
Format Journal Article
LanguageEnglish
Published Chicago, IL American Society of Clinical Pathologists 01.03.1986
Subjects
Analytical, structural and metabolic biochemistry
Bilirubin - blood
Biological and medical sciences
Fundamental and applied biological sciences. Psychology
Hemoglobins - analysis
Hemoproteins
Humans
Metalloproteins
Myoglobin - blood
Plasma - analysis
Proteins
Reference Standards
Spectrophotometry, Ultraviolet
Assay
Human
Hemoprotein
Derivative spectrometry
Clinical biology
Hemoglobin
Technique
Blood
Blood plasma
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Summary:Recording derivative spectrophotometry (DS) is a technic for analyzing absorbance curves by measuring the derivative (slope) of those curves. The derivative is a mathematical function that enhances rapid changes of direction of a curve and suppresses slow changes. We are presenting a simple, rapid, and reproducible method for determining plasma hemoglobin concentration, even in the presence of bilirubin, myoglobin, or marked turbidity. No toxic reagents are used and, since the analysis is purely physicomathematic, the sample is not altered and can be used for additional tests.
ISSN:0002-9173
1943-7722
DOI:10.1093/ajcp/85.3.342