lincRNA-RP11400K9.4 Regulates Cell Survival and Migration of Breast Cancer Cells

Background/Aim: Several works in the past decades pointed out the key role of long intergenic non-coding RNA (lincRNA) in breast cancer development. Here in we report for first time the importance of deregulation of lincRNA RP11-400K9.4 in breast cancer cells which played a role in cell survival and...

Full description

Saved in:
Bibliographic Details
Published inCancer genomics & proteomics Vol. 17; no. 6; pp. 769 - 779
Main Authors FERNÁNDEZ-ROJAS, MIGUEL A., MELENDEZ-ZAJGLA, JORGE, LAGUNAS, VILMA MALDONADO
Format Journal Article
LanguageEnglish
Published Athens International Institute of Anticancer Research 01.11.2020
Subjects
Breast cancer
Cell culture
Cell migration
Cell survival
Deoxyribonucleic acid
Deregulation
DNA
DNA damage
DNA microarrays
DNA repair
Irradiation
Non-coding RNA
Polymerase chain reaction
Radiation damage
Radiation therapy
Resistance factors
Survival
Ultraviolet radiation
Online AccessGet full text

Cover

More Information
Summary:Background/Aim: Several works in the past decades pointed out the key role of long intergenic non-coding RNA (lincRNA) in breast cancer development. Here in we report for first time the importance of deregulation of lincRNA RP11-400K9.4 in breast cancer cells which played a role in cell survival and migration. Materials and Methods: After RP11-400K9.4 silencing by short hairpin RNAs or overexpression by GeneBlocks, real-time quantitative polymerase chain reaction (RT-PCR), microarray, migration, proliferation and viability assay were performed. Results: RP11-400K9.4 expression was mainly in the cytoplasmic fraction in 2D culture. Overexpression of RP11-400K9.4 led to a reduction of migration by MCF-7 and MDA-MB-368 cells and an increase in cellular survival after UV-C radiation. Bioinformatic analyses highlighted irradiation-induced DNA damage, DNA repair and cell-cycle pathways as the mainly affected by RP11-400K9.4. Furthermore RT-PCR assay demonstrated the overexpression of baculoviral IAP repeat containing 3 (BIRC3) a known oncogene that promotes radiotherapy resistance through the nuclear factor kappa B (NFĸB) pathway. Conclusion: RP11-400K9.4 participates in the modulation of migration and survival processes probably via the BIRC3/NFĸB pathway.
ISSN:1109-6535
1790-6245
DOI:10.21873/cgp.20231