GABAA receptor-mediated input change on orexin neurons following sleep deprivation in mice

Highlights • GABAA R α1 expression is increased in orexin neuron following 6-h sleep deprivation. • Sleep deprivation enhances the sensitivity of orexin neuron to GABAA R agonist. • Inhibitory synapses on orexin neuron are profoundly affected by sleep deprivation.

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Bibliographic Details
Published inNeuroscience Vol. 284; pp. 217 - 224
Main Authors Matsuki, T, Takasu, M, Hirose, Y, Murakoshi, N, Sinton, C.M, Motoike, T, Yanagisawa, M
Format Journal Article
LanguageEnglish
Published United States Elsevier Ltd 22.01.2015
Subjects
Animals
Brain - pathology
Cell Adhesion Molecules, Neuronal - metabolism
Dose-Response Relationship, Drug
GABA
GABA Agents - pharmacology
Gene Expression Regulation - drug effects
Gene Expression Regulation - physiology
Green Fluorescent Proteins - genetics
Green Fluorescent Proteins - metabolism
In Vitro Techniques
Inhibitory Postsynaptic Potentials - drug effects
Inhibitory Postsynaptic Potentials - genetics
insomnia
Mice
Mice, Transgenic
Nerve Tissue Proteins - metabolism
Neurology
Neurons - drug effects
Neurons - physiology
orexin/hypocretin
Orexins - genetics
Orexins - metabolism
Patch-Clamp Techniques
receptor
Receptors, GABA-A - metabolism
Sleep Deprivation - pathology
Sleep Deprivation - physiopathology
sleep homeostasis
Wakefulness - physiology
NLGN2
GABA A receptor
lateral hypothalamus
NREM
CNS
melanin-concentrating hormone
PFA
SD
non-rapid eye movement
BZDs
TTX
paraformaldehyde
GABA AR
neuroligin 2
eGFP
benzodiazepines
mIPSCs
miniature inhibitory post synaptic currents
sleep homeostasis
Huntingtin-associated protein 1
ACSF
artificial cerebrospinal fluid
GABA B receptor
central nervous system
insomnia
GABA BR
sleep deprivation
MCH
receptor
enhanced green fluorescent protein
γ-aminobutyric acid
GABA
orexin/hypocretin
SEM
HAP1
standard error of the mean
tetrodotoxin
LH
GABAAR
GABABR
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Summary:Highlights • GABAA R α1 expression is increased in orexin neuron following 6-h sleep deprivation. • Sleep deprivation enhances the sensitivity of orexin neuron to GABAA R agonist. • Inhibitory synapses on orexin neuron are profoundly affected by sleep deprivation.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0306-4522
1873-7544
DOI:10.1016/j.neuroscience.2014.09.063