Study of mitochondrial swelling, membrane fluidity and ROS production induced by nano-TiO2 and prevented by Fe incorporation

Abstract The potential impact of TiO2 and Fe incorporated TiO2 nanoparticles at the organelle level has been reported. The toxicity of the samples on mitochondria isolated from chicken liver tissue has been examined through mitochondrial swelling, membrane fluidity, ROS generation capacity, and acti...

Full description

Saved in:
Bibliographic Details
Published inToxicology research (Cambridge) Vol. 8; no. 5; pp. 711 - 722
Main Authors Barkhade, Tejal, Mahapatra, Santosh Kumar, Banerjee, Indrani
Format Journal Article
LanguageEnglish
Published Cambridge Royal Society of Chemistry 01.09.2019
Subjects
Animal tissues
Anisotropy
Biocompatibility
Chemistry
Fluidity
Iron
Membrane fluidity
Mitochondria
Morphology
Nanoparticles
Poultry
Succinate dehydrogenase
Swelling
Titanium dioxide
Toxicity
Viscosity
Online AccessGet full text

Cover

More Information
Summary:Abstract The potential impact of TiO2 and Fe incorporated TiO2 nanoparticles at the organelle level has been reported. The toxicity of the samples on mitochondria isolated from chicken liver tissue has been examined through mitochondrial swelling, membrane fluidity, ROS generation capacity, and activity of complex II. The toxic effect of TiO2 was prevented by incorporating Fe into the TiO2 matrix at different concentrations. The activity of the succinate dehydrogenase enzyme complex was affected and permeabilization of the mitochondrial inner membrane to H+ and K+ and its alteration in membrane fluidity at 100 μg mL−1 of nano-TiO2 dosage were investigated, which showed significant changes in the anisotropy of DPH-labeled mitochondria. Fe incorporation into the TiO2 matrix makes it more biocompatible by changing its structure and morphology.
ISSN:2045-4538
2045-452X
2045-4538
DOI:10.1039/c9tx00143c