Aptamer-based determination of ATP by using a functionalized impedimetric nanosensor and mediation by a triangular junction transducer

The authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM conc...

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Bibliographic Details
Published inMikrochimica acta (1966) Vol. 184; no. 11; pp. 4425 - 4431
Main Authors Gopinath, Subash C. B., Perumal, Veeradasan, Balakrishnan, S.R., Md Arshad, M. K., Lakshmipriya, Thangavel, Haarindraprasad, R., Hashim, Uda
Format Journal Article
LanguageEnglish
Published Vienna Springer Vienna 01.11.2017
Springer
Springer Nature B.V
Subjects
Adenosine triphosphate
Alternating current
Analytical Chemistry
Biotin
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Consumer goods
Dielectric properties
Dilution
Engineering
Mediation
Microengineering
Nanochemistry
Nanosensors
Nanotechnology
Original Paper
Sensors
Signal to noise ratio
Urine
Urogenital system
Biotinylation
ATP aptamer
Aptasensor
Micro-device
Human urine
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Summary:The authors describe an impedimetric nanosensor for aptamer-mediated detection of ATP. A triangular junction of polysilicon substrate is used as a transducer. The aptamer was immobilized on the substrate via biotin-streptavidin interaction. ATP can be detected by this method in the 10 to 100 nM concentration range at room temperature and estimated the signal to noise ratio above the range of 3σ. It was applied to the detection of ATP in 1:1000 diluted human urine. The desired transducer is highly reproducible and specific. It is selective over closely related molecules such as guanosine triphosphate, cytidine triphosphate and uridine triphosphate. The method is perceived to represent a valuable tool for the diagnosis for infections and inflammations. The novelties shown in this study compared to other sensors are, consumption of low sample volume (~ 1 μl), non-invasive and suitable with AC and DC currents. Graphical abstract Aptamer-functionalized Impedimetric Nanosensor for ATP Determination in Human Urine by a Triangular Junction Transducer.
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-017-2485-8