A solid-phase radioimmunoassay for fibrinogen

A solid-phase radioimmunoassay for fibrinogen has been developed utilizing [ 14C]-methylated fibrinogen as standard antigen and fibrinogen-specific antibodies covalently linked to Sepharose. Fibrinogen was [ 14C]-methylated by reductive alkylation using [ 14C] formaldehyde and sodium borohydride. Th...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 64; no. 1; pp. 60 - 67
Main Authors Crane, Laura J., Miller, David L.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.03.1975
Subjects
Animals
Antibodies - isolation & purification
Binding Sites, Antibody
Blood
Blood Coagulation
Fibrinogen - analysis
Fibrinogen - immunology
Hydrogen-Ion Concentration
Male
Methylation
Rabbits - immunology
Radioimmunoassay
Radioisotope Dilution Technique
Rats
Sepharose
Time Factors
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Summary:A solid-phase radioimmunoassay for fibrinogen has been developed utilizing [ 14C]-methylated fibrinogen as standard antigen and fibrinogen-specific antibodies covalently linked to Sepharose. Fibrinogen was [ 14C]-methylated by reductive alkylation using [ 14C] formaldehyde and sodium borohydride. The methylated fibrinogen was unaltered in clotting ability and antigenicity. The assay, an isotope dilution assay, is quantitative for picomole amounts of fibrinogen. It is specific for fibrinogen in homologous plasma and in the presence of a variety of other proteins.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(75)90404-2