Proton NMR Spectroscopy of the Phospholipids in Human Uterine Smooth Muscle and Placenta

• Published in: Gynecologic and obstetric investigation Vol. 46; no. 4; pp. 220 - 224
• Main Authors: Pulkkinen, Martti O., Nyman, Samuel, Hämäläinen, Mauri M., Mattinen, Jorma
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Karger 01.01.1998; S. Karger AG
• Subjects: Adult; Biological and medical sciences; Butanols; Cholesterol - analysis; Fatty Acids - analysis; Fatty Acids - chemistry; Female; Fundamental and applied biological sciences. Psychology; Humans; Labor, Obstetric - physiology; Magnetic Resonance Spectroscopy; Membrane Fluidity; Mother. Fetoplacental unit. Mammary gland. Milk; Muscle, Smooth - chemistry; Myometrium - chemistry; Original Paper; Phospholipids - analysis; Phospholipids - chemistry; Placenta - chemistry; Pregnancy; Pregnancy. Parturition. Lactation; Vertebrates: reproduction; Proton NMR; Uterine muscle; Phospholipids; Placenta; Human; Phospholipid; Lipids; Exploration; Smooth muscle; Nuclear magnetic resonance imaging; Cholesterol; Pregnancy; Spectrometry; Fluidity; Uterus; Proton; Work; Medical imagery; Female; Physiology
• ISSN: 0378-7346; 1423-002X
• DOI: 10.1159/000010038

The parameters regulating the fluidity of myometrial and placental phospholipids include double bonds, fatty acid chain lenght and the cholesterol/phospholipid ratio. The transformation of these parameters was studied during pregnancy and labor. Myometrial and placental tissue samples were collected from 24 patients: 6 were nonpregnant, 6 early-pregnant, 6 late-pregnant not in labor and 6 in labor. After butanol extraction, tissue cholesterol and lipid phosphorus were determined. Proton NMR spectroscopy of the phospholipids was performed at 500 MHz. The myometrial cholesterol/phospholipid ratio was slightly elevated in pregnant patients not in labor. The uterine muscle of the nonpregnant patients contained more CH=CH groups in the phospholipids than that of the late-pregnant patients. There were 29 more double bonds in placental than in uterine tissue per 100 fatty acid molecules. The average fatty acid chain length varied from 14.0 to 18.8. The placenta has longer fatty acid chains than the uterine smooth muscle. The myometrial carbon chain was shortened on the average by 1.4 and the placental by 1.0 carbon atoms, when the patient went into clinical labor. These findings suggest fluidity changes in myometrial and placental phospholipids during human pregnancy and labor.