RNA-seq reveals mechanisms of SlMX1 for enhanced carotenoids and terpenoids accumulation along with stress resistance in tomato

• Published in: Science bulletin Vol. 62; no. 7; pp. 476 - 485
• Main Authors: Ewas, Mohamed, Gao, Yangqiang, Ali, Farhan, Nishawy, Elsayed M., Shahzad, Raheel, Subthain, Hizar, Amar, Mohamed, Martin, Cathie, Luo, Jie
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.04.2017
• Subjects: Carotenoids; Mechanism; Resistance; RNA Seq; SlMX1; Terpenoids; Tomato; Resistance; Carotenoids; Terpenoids; RNA Seq; Tomato; SlMX1; Mechanism
• ISSN: 2095-9273; 2095-9281
• DOI: 10.1016/j.scib.2017.03.018

Improving nutritional fruit quality and impacts important agro-traits such as biotic or abiotic stresses are extremely important for human civilization. Our previous study reported that manipulation of SlMX1 gene enhanced carotenoids accumulation and drought resistance in tomato. Here, RNA-Seq analysis proved to be a very useful tool to provide insights into the regulatory mechanisms of SlMX1 involved in stress resistance and enhanced secondary metabolites. Physiological analysis showed that over- expression of SlMX1 results in substantially increased broad-spectrum tolerance to a wide-range of abiotic and biotic （fungus, bacteria, virus and insects） stresses in tomato. This research appears to be of remarkable interest because enhanced terpenoids content has been achieved by increasing trichome density. In addition, we reported two types of trichome which seems to be aberrant types in tomato. This study unravels the mechanism of regulation of SlMX1, which simultaneously modulates resistance and metabolic processes through regulating key structural and regulatory genes of the corresponding pathways.