Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner
A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to infor...
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Published in | Conservation genetics resources Vol. 6; no. 3; pp. 689 - 691 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer-Verlag
01.09.2014
Springer Netherlands Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles. |
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Bibliography: | http://dx.doi.org/10.1007/s12686-014-0183-y |
ISSN: | 1877-7252 1877-7260 |
DOI: | 10.1007/s12686-014-0183-y |