Characterisation of microsatellite DNA markers for Grevillea globosa C. A. Gardner

A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to infor...

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Bibliographic Details
Published inConservation genetics resources Vol. 6; no. 3; pp. 689 - 691
Main Authors Millar, Melissa A, Byrne, Margaret, Coates, David J, Roberts, J. Dale
Format Journal Article
LanguageEnglish
Published Dordrecht Springer-Verlag 01.09.2014
Springer Netherlands
Springer Nature B.V
Subjects
Alleles
Animal Genetics and Genomics
Biodiversity
Biomedical and Life Sciences
Conservation Biology/Ecology
Ecology
Evolutionary Biology
Genetic markers
Genetic structure
genomic libraries
Grevillea
Heredity
Life Sciences
loci
Microsatellite Letters
microsatellite repeats
Microsatellites
null alleles
Plant Genetics and Genomics
Population genetics
shrubs
Western Australia
Western Australia
M13 tagged primer
Microsatellites
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Summary:A genomic library was constructed and 14 novel polymorphic di- and tri-nucleotide nuclear microsatellite markers were developed for Grevillea globosa, an endemic shrub of southwest Western Australia. Populations are patchily distributed and population genetic structure is being investigated to inform appropriate seed collection and restoration strategies. Diversity in a selected population was high, with the number of alleles per locus ranging from 3 to 13 and expected and observed heterozygosities averaging 0.693 and 0.799 respectively. All loci showed independent inheritance and there was no evidence of possible null alleles.
Bibliography:http://dx.doi.org/10.1007/s12686-014-0183-y
ISSN:1877-7252
1877-7260
DOI:10.1007/s12686-014-0183-y