Identification of Dioscorea opposite Thunb. CDPK gene family reveals that DoCDPK20 is related to heat resistance
Temperature affects the growth and yield of yam ( Dioscorea opposite Thunb.), and calcium-dependent protein kinases (CDPKs) play an important role in the plant stress response. However, there has been a lack of system analyses of yam’s CDPK gene family. In this study, 29 CDPK transcriptome sequences...
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Published in | PeerJ (San Francisco, CA) Vol. 11; p. e16110 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
San Diego, USA
PeerJ Inc
20.09.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Temperature affects the growth and yield of yam (
Dioscorea opposite
Thunb.), and calcium-dependent protein kinases (CDPKs) play an important role in the plant stress response. However, there has been a lack of system analyses of yam’s
CDPK
gene family. In this study, 29
CDPK
transcriptome sequences with complete open reading frames (ORFs) were identified from yam RNA sequencing data. The sequences were classified into four groups (I–VI) using phylogenetic analysis. Two
DoCDPK
genes were randomly selected from each group and the gene patterns of yam leaves were determined using quantitative real-time PCR (qRT-PCR) under high and low temperature stress in order to show their unique functions in mediating specific responses. Among them,
DoCDPK20
was significantly induced in high temperatures. The pPZP221-
DoCDPK20
was transformed into tobacco leaves using an agrobacterium-mediated method. Under high temperature stress,
DoCDPK20
overexpression reduced photosynthesis and improved heat tolerance in transgenic tobacco. Our research offers meaningful perspectives into
CDPK
genes and new avenues for the genetic engineering and molecular breeding of yam. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 2167-8359 2167-8359 |
DOI: | 10.7717/peerj.16110 |