Structural characterization of sulfated galactofucan from Undaria pinnatifida and its effect on type 2 diabetic mice

• Published in: Journal of oceanology and limnology Vol. 41; no. 1; pp. 300 - 313
• Main Authors: Ke, Songze, Zhang, Bo, Yu, Yanlei, Wang, Sijia, Jin, Weihua, Wu, Jian, Chen, Jianwei, Zhang, Huawei, Wei, Bin, Wang, Hong
• Format: Journal Article
• Language: English
• Published: Heidelberg Science Press 01.01.2023; Springer Nature B.V; College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Moganshan Research Institute at Deqing County Zhejiang University of Technology,Zhejiang University of Technology,Hangzhou 310014,China%College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Moganshan Research Institute at Deqing County Zhejiang University of Technology,Zhejiang University of Technology,Hangzhou 310014,China; Center for Human Nutrition,David Geffen School of Medicine,University of California,Los Angeles CA 90024,USA%College of Biotechnology and Bioengineering,Zhejiang University of Technology,Hangzhou 310014,China%Zhejiang Provincial Key Laboratory of Traditional Chinese Medicine Pharmaceutical Technology,Hangzhou 310014,China; Key Laboratory of Marine Fishery Resources Exploitment&Utilization of Zhejiang Province,Hangzhou 310014,China%College of Pharmaceutical Science&Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals,Moganshan Research Institute at Deqing County Zhejiang University of Technology,Zhejiang University of Technology,Hangzhou 310014,China
• Subjects: Abundance; acid hydrolysis; Algae; Bilophila; Biology; blood glucose; blood lipids; Calcium; Calcium chloride; Chemical analysis; Diabetes; Diabetes mellitus; Diabetes mellitus (non-insulin dependent); Earth and Environmental Science; Earth Sciences; Ethanol; Fucose; Galactose; Homeostasis; Intestinal flora; Intestinal microflora; intestinal microorganisms; Limnology; Lipids; Metabolism; Microbiota; Molecular weight; NMR; noninsulin-dependent diabetes mellitus; Nuclear magnetic resonance; Nutrition research; Oceanography; R&D; Research & development; Saccharides; Seaweeds; Serum; Structural analysis; sulfates; Sulfation; Sulfuric acid; Undaria pinnatifida; galactofucan; diabetes; structure; gut microbiota; Undaria pinnatifida
• ISSN: 2096-5508; 2523-3521
• DOI: 10.1007/s00343-021-1307-3

A sulfated galactofucan designated as UpG was obtained from the brown algae Undaria pinnatifida by calcium chloride extraction. Chemical analyses showed that UpG is composed of galactose and fucose at a high sulfation level. Low-molecular weight UpGP-0.5 was prepared from UpG through acid hydrolysis for structure characterization. The backbones of UpG are determined to be α-(1,3)-Fuc, α-(1,4)-Gal, α-(1,3)-Gal, and α-(1,6)-Gal by GC-MS, FT-IR, NMR, and LC-MS analyses. Sulfate groups are modified at C2 and/or C4 of fucose and C3 and/or C4 of galactose. UpG could partially lower blood sugar and serum lipid levels in type 2 diabetic mice. Moreover, UpG treatment regulates the abundance of some specific gut microbiota, such as enriching the abundance of Muribaculum and Christensenellaceae, and reducing that of Bilophila , Tannerellaceae, Candidatus Saccharimonas and Anaerotruncus . The findings characterized the detailed structure of a sulfated galactofucan and investigated its potential for the treatment of type 2 diabetes mellitus.