Imaging living central neurones using viral gene transfer

Studies of central neurones and other cellular components of the brain, such as glial and vascular cells, can be greatly advanced by the use of the modern optical techniques such as confocal live cell imaging. Fluorescent proteins have allowed imaging of particular cell types or intracellular elemen...

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Bibliographic Details
Published inAdvanced drug delivery reviews Vol. 57; no. 1; pp. 79 - 93
Main Authors Teschemacher, A.G., Paton, J.F.R., Kasparov, S.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 02.01.2005
Subjects
Adenoviridae - genetics
Adenovirus
Animals
Brain - metabolism
Brain - virology
Cells, Cultured
Fluorescent Antibody Technique - methods
Gene expression
Gene Expression Regulation, Viral - genetics
Gene Expression Regulation, Viral - physiology
Imaging
Lentivirus
Lentivirus - genetics
Mice
Molecular Biology - methods
Neurones
Neurons - virology
Organotypic cultures
Phenotype
Rats
Signal Transduction - genetics
Viral vectors
Neurones
Organotypic cultures
Adenovirus
Imaging
Viral vectors
Gene expression
Lentivirus
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Summary:Studies of central neurones and other cellular components of the brain, such as glial and vascular cells, can be greatly advanced by the use of the modern optical techniques such as confocal live cell imaging. Fluorescent proteins have allowed imaging of particular cell types or intracellular elements to be visualised and distinguished from irrelevant background structures. To introduce the genetic information encoding for fluorescent proteins into relevant cellular targets, molecular tools are required. Viral vectors are one of the best ways of gene delivery into differentiated postnatal brain neurones and glia. Current progress in this field allows targeting of various cell types and therefore makes it possible to express a variety of fluorescent constructs in selected subpopulations of neurones, for example. In this review, we will discuss and compare the properties of the most popular viral gene delivery systems and the advantages of different brain cell preparations to illustrate how they can be used for high-resolution live cell confocal imaging in order to study new aspects of central nervous system (CNS) structure and function.
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ISSN:0169-409X
1872-8294
DOI:10.1016/j.addr.2004.05.004