Production of 1,3-Propanediol from Glucose by Recombinant Escherichia coli BL21(DE3)

• Published in: Biotechnology and bioprocess engineering Vol. 23; no. 2; pp. 250 - 258
• Main Authors: Lee, Jae Hyeon, Lama, Suman, Kim, Jung Rae, Park, Sung Hoon
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.03.2018; Springer Nature B.V; 한국생물공학회
• Subjects: 1,3-Propanediol; acetates; Acetic acid; Aldolase; Bacteria; Biotechnology; carbon; Chemistry; Chemistry and Materials Science; Dehydration; Dihydroxyacetone; Dihydroxyacetone phosphate; E coli; Entner-Doudoroff pathway; Escherichia coli; fructose-bisphosphate aldolase; Glucose; Glycerol; Industrial and Production Engineering; phosphates; Phosphogluconate dehydratase; Research Paper; toxicity; 생물공학; BL21(DE3); pathway engineering; 1,3- PDO; glycerol
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-018-0017-y

A range of recombinant strains of Escherichia coli were developed to produce 1,3-propanediol (1,3-PDO), an important C3 diol, from glucose. Two modules, the glycerol-producing pathway converting dihydroxyacetone phosphate to glycerol and the 1,3-PDO-producing pathway converting glycerol to 1,3-PDO, were introduced into E. coli . In addition, to avoid oxidative assimilation of the produced glycerol, glycerol oxidative pathway was deleted. Furthermore, to enhance the carbon flow to the Embden- Meyerhof-Parnas pathway, the Entner-Doudoroff pathway was disrupted by deleting 6-phosphogluconate dehydratase and 2-keto-3-deoxy-6-phosphogluconate aldolase. Finally, the acetate production pathway was removed to minimize the production of acetate, a major and toxic by-product. Flask experiments were carried out to examine the performance of the developed recombinant E. coli . The best strain could produce 1,3-PDO with a yield of 0.47 mol/mol glucose. Along with 1,3-PDO, glycerol was produced with a yield of 0.33 mol/mol glucose.