Direct plantlet inoculation with soil or insect-associated fungi may control cabbage root fly maggots

• Published in: Journal of invertebrate pathology Vol. 120; pp. 59 - 66
• Main Authors: Razinger, Jaka, Lutz, Matthias, Schroers, Hans-Josef, Palmisano, Marilena, Wohler, Christian, Urek, Gregor, Grunder, Jürg
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01.07.2014; Elsevier
• Subjects: Animals; Biological and medical sciences; Biological control; Brassica - microbiology; Control; Delia radicum; Diptera; Diptera - microbiology; Entomopathogenic fungi; Fundamental and applied biological sciences. Psychology; Fungi; Larva - microbiology; Pest Control, Biological - methods; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; Plant Roots - growth & development; Plant Roots - microbiology; Protozoa. Invertebrates; Rhizosphere competence; Soil pest; Delia radicum; Entomopathogenic fungi; Rhizosphere competence; Soil pest; Biological control; Diptera; Entomopathogen; Insecta; Fungi; Rhizosphere; Soils; Inoculation; Pest; Arthropoda; Cauliflower; Invertebrata; Anthomyiidae
• ISSN: 0022-2011; 1096-0805
• DOI: 10.1016/j.jip.2014.05.006

Photomicrograph of Metarhizium anisopliae 1154 growing on cauliflower rhizoplane (left), stained with cotton blue. Cabbage root fly (Delia radicum L.) larva infected with M. anisopliae 1154 (right). [Display omitted] •Inoculation with fungi reduced the number of cabbage root fly pupae.•All fungal species tested were rhizosphere competent to cauliflower roots.•The tested fungi could be transferred via or grow with the elongating roots.•Young plantlet inoculation may be a promising biological control strategy. A potential Delia radicum biological control strategy involving cauliflower plantlet inoculation with various fungi was investigated in a series of laboratory and glasshouse experiments. In addition to entomopathogenic fungi, fungi with a high rhizosphere competence and fungi with the ability to survive as saprotrophs in soil were tested. The following fungal species were evaluated in the experiments: Trichoderma atroviride, T. koningiopsis, T. gamsii, Beauveria bassiana, Metharhizium anisopliae, M. brunneum and Clonostachys solani. A commercial carbosulfan-based insecticide was used as a positive control. Additionally, two commercial products, one based on B. bassiana (Naturalis) and one on Bacillus thuringiensis (Delfin) were used as reference biocontrol agents. The aims were (i) to assess the pathogenicity of the selected fungal isolates to Delia radicum, (ii) to evaluate the fungal isolates’ rhizosphere competence, with the emphasis on the persistence of the original inoculum on the growing roots, (iii) to assess possible endophytic plant tissue colonization, and (iv) to evaluate potential plant growth stimulating effects of the added inoculi. Significant pathogenicity of tested fungi against Delia radicum was confirmed in in vitro and glasshouse experiments. All tested fungi persisted on cauliflower rhizoplane. More importantly, the added fungi were found on thoroughly washed roots outside the original point of inoculation. This provided us with evidence that our tested fungi could be transferred via or grow with the elongating roots. In addition to colonizing the rhizoplane, some fungi were found inside the plant root or stem tissue, thus exhibiting endophytic characteristics. The importance of fungal ecology as a criterion in appropriate biological control agent selection is discussed.