Integration and organization of Ti plasmid sequences in crown gall tumors

The Ti plasmid sequences (T DNA) maintained in four unorganized crown gall tumor lines were defined by using restriction endonucleases and molecular hybridization techniques. Each tumor line contains a “core” T DNA which is apparently responsible for maintaining the transformed state; the core T DNA...

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Bibliographic Details
Published inCell Vol. 19; no. 3; pp. 729 - 739
Main Authors Thomashow, Michael F., Nutter, Robert, Montoya, Alice L., Gordon, Milton P., Nester, Eugene W.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.01.1980
Subjects
Base Sequence
DNA - genetics
DNA, Bacterial - genetics
Nucleic Acid Hybridization
Plant Tumors - microbiology
Plasmids
Recombination, Genetic
Rhizobium - genetics
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Summary:The Ti plasmid sequences (T DNA) maintained in four unorganized crown gall tumor lines were defined by using restriction endonucleases and molecular hybridization techniques. Each tumor line contains a “core” T DNA which is apparently responsible for maintaining the transformed state; the core T DNA is colinear with the Ti plasmid and contains the Ti plasmid sequences referred to as the “common DNA”—sequences found in all Ti plasmids studied to date. A given Ti plasmid does not always give rise to the same T DNA complement. The data suggest that the majority if not all of the T DNA is integrated into plant DNA, that preferred regions of the Ti plasmid serve as the points of attachment to plant DNA and that the T DNA can be linked to more than one site in the plant genome.
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H20
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ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(80)80049-3