Cytochemical location of urease in the cell wall of two different lichen phycobionts

The enzyme urease has been located in the cell wall of recently isolated phycobionts from Evernia prunastri and Xanthoria parietina lichens. Cytochemical detection is achieved by producing a black, electron-dense precipitate of cobalt sulfide proceeding from CO 2 evolved from urea in the presence of...

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Published inTissue & cell Vol. 36; no. 6; pp. 373 - 377
Main Authors Millanes, A.M., Fontaniella, B., García, M.L., Solas, M.T., Vicente, C., Legaz, M.E.
Format Journal Article
LanguageEnglish
Published Scotland Elsevier Ltd 01.12.2004
Subjects
Cell Fractionation
Cell wall
Cell Wall - enzymology
Cell Wall - ultrastructure
Eukaryota - enzymology
Eukaryota - ultrastructure
Evernia prunastri
Lichens - enzymology
Lichens - ultrastructure
Microscopy, Electron, Transmission
Phycobiont
Symbiosis - physiology
Urease
Urease - metabolism
Xanthoria parietina
Urease
Xanthoria parietina
Evernia prunastri
Phycobiont
Cell wall
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Summary:The enzyme urease has been located in the cell wall of recently isolated phycobionts from Evernia prunastri and Xanthoria parietina lichens. Cytochemical detection is achieved by producing a black, electron-dense precipitate of cobalt sulfide proceeding from CO 2 evolved from urea in the presence of cobalt chloride. Cellular fractionation reveals that about 80% of total urease activity was associated to the cell wall on both phycobionts whereas only 20% was recovered as soluble protein.
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ISSN:0040-8166
1532-3072
DOI:10.1016/j.tice.2004.06.007