Effects of Allopurinol on Renal Stone Formation and Osteopontin Expression in a Rat Urolithiasis Model

Background: The inhibitory effect of allopurinol on calcium oxalate urolithiasis has been reported, but its effect on stone matrix proteins has not been studied in vivo. To clarify the effect of allopurinol on the matrix, we investigated its effect on the expression of osteopontin (OPN), which we pr...

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Bibliographic Details
Published inNephron Vol. 87; no. 2; pp. 170 - 176
Main Authors Yasui, Takahiro, Sato, Motohiko, Fujita, Keiji, Ito, Yasunori, Nomura, Shintaro, Kohri, Kenjiro
Format Journal Article
LanguageEnglish
Published Basel, Switzerland S. Karger AG 01.02.2001
Subjects
Allopurinol - pharmacology
Animals
Calcium Oxalate - metabolism
Gene Expression - drug effects
Immunohistochemistry
In Situ Hybridization
Kidney - drug effects
Kidney - metabolism
Kidney - pathology
Kidney Calculi - genetics
Kidney Calculi - metabolism
Kidney Calculi - prevention & control
Male
Original Paper
Osteopontin
Rats
Rats, Wistar
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sialoglycoproteins - genetics
Sialoglycoproteins - metabolism
Osteopontin
Ethylene glycol
Calcium oxalate
Urolithiasis
Allopurinol
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Summary:Background: The inhibitory effect of allopurinol on calcium oxalate urolithiasis has been reported, but its effect on stone matrix proteins has not been studied in vivo. To clarify the effect of allopurinol on the matrix, we investigated its effect on the expression of osteopontin (OPN), which we previously identified as an important stone matrix protein. Methods: Control rats were not treated. Rats of the stone group were given ethylene glycol (EG) and vitamin D 3 , while the allopurinol groups (low-dose group and high-dose group) were treated with allopurinol in addition to receiving EG and vitamin D 3 . Results: The rate of renal stone formation was lower in the allopurinol groups than in the stone group. This was associated with a low expression of OPN mRNA in allopurinol-treated rats relative to that in the stone group. Conclusion: Allopurinol was effective in preventing calcium oxalate stone formation and reduced OPN expression in rats. Our results suggest that allopurinol prevents renal stone formation by acting against not only the control of oxalate but also OPN expression.
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ISSN:1660-8151
2235-3186
DOI:10.1159/000045907