CXCL8 and CXCL11 chemokine secretion in dermal fibroblasts is differentially modulated by vanadium pentoxide

• Published in: Molecular medicine reports Vol. 18; no. 2; pp. 1798 - 1803
• Main Authors: Fallahi, P., Foddis, R., Elia, G., Ragusa, F., Patrizio, A., Benvenga, S., Cristaudo, A., Antonelli, A., Ferrari, S.
• Format: Journal Article
• Language: English
• Published: Greece Spandidos Publications 01.08.2018; Spandidos Publications UK Ltd
• Subjects: Aged; Atopic dermatitis; Bronchitis; Care and treatment; Cell proliferation; Cell Proliferation - drug effects; Chemokine CXCL11 - genetics; Chemokines; CXCL11 protein; Cytokines; Dermatitis; Development and progression; Enzyme-linked immunosorbent assay; Experiments; Female; Fibroblasts; Fibroblasts - drug effects; Gene expression; Gene Expression Regulation - drug effects; Genetic aspects; Health aspects; Humans; Immune response; Inflammation; Inflammation - drug therapy; Inflammation - genetics; Inflammation - pathology; Interferon-gamma - administration & dosage; Interleukin-8 - genetics; Middle Aged; Occupational safety; Skin; Studies; Thyroid gland; Tumor necrosis factor-α; Vanadium; Vanadium Compounds - pharmacology; Vanadium pentoxide; Variance analysis; γ-Interferon; United States > US
• ISSN: 1791-2997; 1791-3004; 1791-3004
• DOI: 10.3892/mmr.2018.9121

An increase in skin rashes or atopic dermatitis has been observed in individuals working with vanadium. However, to the best of our knowledge no in vivo or in vitro studies have evaluated the effect of exposure to vanadium in dermal fibroblasts. Cells viability and proliferation were assessed by WST‑1 assay, cells were treated with increasing concentrations of V2O5 (1, 10 and 100 nM). CXCL8 and CXCL11 concentrations were measured in the supernatants using an ELISA assay. V2O5 was not observed as having a significant effect on dermal fibroblast's viability and proliferation. However, it was revealed that V2O5 was able to induce the secretion of CXCL8 and CXCL11 chemokines into dermal fibroblasts. V2O5 synergistically increased the effect of interferon (IFN)γ on CXCL11 secretion. In addition, V2O5 synergistically increased the effect of the tumor necrosis factor α on CXCL8 secretion and abolished the inhibitory effect of IFNγ. V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the appearance and perpetuation of an inflammatory reaction into the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in subjects occupationally exposed, or living in polluted areas.