Conservation of CD1 protein expression patterns in the chicken

• Published in: Developmental and comparative immunology Vol. 34; no. 2; pp. 123 - 132
• Main Authors: Ly, Nary, Danzl, Nicole M., Wang, Jing, Zajonc, Dirk M., Dascher, Christopher C.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.02.2010
• Subjects: Animals; Antibody; Antigens, CD1 - immunology; Antigens, CD1 - metabolism; Avian; Avian Proteins - immunology; Avian Proteins - metabolism; CD1; Cell Line; Chick Embryo; Chickens - growth & development; Chickens - immunology; Chickens - metabolism; Dendritic cells; Evolution; Humans; Lipid; Lymphoid Tissue - embryology; Lymphoid Tissue - growth & development; Lymphoid Tissue - immunology; MHC; Protein Transport; CD1; Dendritic cells; Antibody; Evolution; MHC; Avian; Lipid
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2009.08.015

The CD1 molecules are cell-surface proteins that bind and present foreign lipids and glycolipids to T cells in a manner similar to the MHC system. While the mammalian CD1 antigen presentation pathway is well characterized, little is known about CD1 in non-mammalian vertebrates. Previous studies have identified two CD1 homologues in the chicken. We developed a monoclonal antibody designated NL1-1.A1 specific for the chCD1-1 isoform and have used this to characterize CD1 expression in tissues and cells of normal adult and embryonic chickens. The chCD1-1 isoform is expressed on a high proportion of cells in the spleen and bursa. Cells in the spleen that stain for CD1 are also positive for IgM and consistent with identification of these as B cells. In the skin, chCD1-1 is expressed on cells with dendritic morphology along the dermal–epidermal boundary and in epidermal sheets consistent with chicken Langerhans cells. Staining of cells in the medullary region of the chicken thymus was also observed. The CD1 proteins in mammals traffic to intracellular compartments to acquire lipid antigens for subsequent presentation to T cells on the surface. Consistent with data from mammal CD1 proteins, chCD1-1 partially co-localized with a lysosomal marker in the myeloid cell line BM2. Taken together, these data support broad distribution of chCD1-1 in both lymphoid and non-lymphoid tissues of the chicken that is remarkably similar to the distribution of CD1 isoforms in mammals.