Genetic and Antibiotic Resistance Characteristics of Campylobacter jejuni Isolated from Diarrheal Patients, Poultry and Cattle in Shenzhen

• Published in: Biomedical and environmental sciences Vol. 31; no. 8; pp. 579 - 585
• Main Authors: JU, Chang Yan, ZHANG, Mao Jun, MA, Yan Ping, LU, Jing Rang, YU, Mu Hua, CHEN, Hui, LIU, Chu Yun, GU, Yi Xin, FU, Yan Yan, DUAN, Yong Xiang
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.08.2018; Nanshan Center for Disease Control and Prevention,Shenzhen 518054,Guangdong,China%State Key Laboratory of Infectious Disease Prevention and Control,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,National Institute for Communicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China%ORD,Environmental Protection Agency,Cincinnati,Ohio 45220,United States
• Subjects: Adolescent; Adult; Animals; Anti-Bacterial Agents - pharmacology; Antibiotic resistance; Campylobacter Infections - microbiology; Campylobacter jejuni; Campylobacter jejuni - drug effects; Campylobacter jejuni - genetics; Campylobacter jejuni - isolation & purification; Cattle; Child; Child, Preschool; China; Diarrhea - microbiology; Diarrheal patients; Drug Resistance, Microbial - genetics; Feces - microbiology; Genotype; Humans; Infant; Microbial Sensitivity Tests; Multilocus Sequence Typing; Poultry; Young Adult; China; Multilocus sequence typing; Poultry; Cattle; Antibiotic resistance; Campylobacter jejuni; Diarrheal patients
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2018.079

To investigate genetic and antibiotic resistance characteristics of Campylobacter jejuni (C. jejuni) isolated from Shenzhen. Multilocs sequence typing and agar dilution methods were used to define the genotype and antibiotic resistance of C. jejuni, respectively. In total, 126 C. jejuni strains were isolated. The prevalence of C. jejuni was 5.3% in diarrheal patients. The prevalence in poultry meat (36.5%) was higher than that in cattle meat (1.1%). However, the prevalence in poultry cloacal swabs (27.0%) was lower than that in cattle stool (57.3%). Sixty-two sequence types were obtained, among which 27 of the STs and 10 alleles were previously unreported. The most frequently observed clonal complexes were ST-21 (11.9%), ST-22 (10.3%), and ST-403 (7.1%). ST-21, ST-45, ST-354, ST-403, and ST-443 complexes overlapped between isolates from patients and cattle, whereas ST-45 and ST-574 complexes overlapped between isolates from patients and poultry. All C. jejuni were resistant to at least one antibiotic. The highest resistance rate was toward ciprofloxacin (89.7%), followed by tetracycline (74.6%), and nalidixic acid (69.0%). This is the first report of the genotypes and antibiotic resistance of C. jejuni in Shenzhen. Overlapping clonal complexes were found between isolates from patients and cattle, and between patients and poultry.