Human granulosa cells contain insulin-like growth factor-binding protein (IGF BP-1) mRNA

• Published in: Clinical endocrinology (Oxford) Vol. 32; no. 5; p. 635
• Main Authors: Koistinen, R, Suikkari, A M, Tiitinen, A, Kontula, K, Seppälä, M
• Format: Journal Article
• Language: English
• Published: England 01.05.1990
• Subjects: Adult; Aged; Carrier Proteins - genetics; Cells, Cultured; Female; Gene Expression; Granulosa Cells - metabolism; Humans; In Vitro Techniques; Insulin-Like Growth Factor Binding Proteins; Middle Aged; Molecular Weight; Nucleic Acid Hybridization; Ovarian Neoplasms - metabolism; Ovary - metabolism; RNA, Messenger - analysis; RNA, Messenger - metabolism
• DOI: 10.1111/j.1365-2265.1990.tb00907.x
• ISSN: 0300-0664

Previous studies have demonstrated expression of insulin-like growth factor-binding protein (IGF BP-1) in secretory and decidualized endometrium, in adult and fetal liver, and in HepG2 liver cancer cells. We have studied the expression of IGF BP-1 in various types of ovarian neoplasias, normal ovary, and granulosa cells from hyperstimulated human ovarian follicles by RNA blot hybridization. A single 1.6 kb mRNA species, similar to that present in human decidua, was identified in poly(A)RNA-containing preparations of granulosa cells and of a borderline malignant ovarian cystadenoma. This finding verifies the postulated production of IGF BP-1 by the human ovary.