Expression of the matrix metalloproteinases 2 and 9 in the rat small intestine during intrauterine and postnatal life

The expressions of matrix metalloproteinases 2 and 9 have been described during the development, as an example in heart and tooth but not in the small intestine yet. In this context, this study aimed to evaluate the expressions of MMP‐2 and MMP‐9 in the small intestine of Wistar rats during intraute...

Full description

Saved in:
Bibliographic Details
Published inAnatomical record (Hoboken, N.J. : 2007) Vol. 303; no. 11; pp. 2839 - 2846
Main Authors dos Reis, Camila Audrey, Miranda Soares, Maria Albertina, Gomes, José Rosa
Format Journal Article
LanguageEnglish
Published Hoboken, USA John Wiley & Sons, Inc 01.11.2020
Wiley Subscription Services, Inc
Subjects
Animals
Birth
development
Epithelial cells
Female
Goblet cells
Goblet Cells - metabolism
Immunohistochemistry
Intestine, Small - embryology
Intestine, Small - metabolism
Lamina propria
Male
Mast cells
Mast Cells - metabolism
Matrix metalloproteinase
Matrix Metalloproteinase 2 - genetics
Matrix Metalloproteinase 2 - metabolism
Matrix Metalloproteinase 9 - genetics
Matrix Metalloproteinase 9 - metabolism
MMP‐2
MMP‐9
Mucous Membrane - metabolism
rat small intestine
Rats
Rats, Wistar
Rodents
Small intestine
rat small intestine
development
MMP-2
mast cells
MMP-9
Online AccessGet full text

Cover

More Information
Summary:The expressions of matrix metalloproteinases 2 and 9 have been described during the development, as an example in heart and tooth but not in the small intestine yet. In this context, this study aimed to evaluate the expressions of MMP‐2 and MMP‐9 in the small intestine of Wistar rats during intrauterine (IU) and postnatal (PN) life. Expressions were determined on the 15th and 18th days of IU life and the 3rd, 10th, 17th, 25th, and 32nd days of PN life. Intestinal samples obtained from six animals were submitted to zymography, immunohistochemistry, and staining with Masson's trichrome. The results showed that MMP‐2 and MMP‐9 were not expressed during IU life; however, after birth, MMP‐9 was immunolocalized in the goblet and mast cells. In conclusion, our results showed that MMP‐2 and MMP‐9 were not expressed in absorptive epithelial cells during the IU period of the small intestine but after birth, MMP‐9 was expressed in the goblet cells, and mast cells present in the lamina propria.
Bibliography:Funding information
Coordination for the Improvement of Higher Education Personnel (CAPES); National Council for Scientific and Technological Development (CNPq), Brazil
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1932-8486
1932-8494
DOI:10.1002/ar.24314