A new nuclear DNA marker from ubiquitin ligase gene region for genetic diversity detection of walnut germplasm resources

• Published in: Biotechnology reports (Amsterdam, Netherlands) Vol. 5; no. C; pp. 40 - 45
• Main Authors: Suo, Zhili, Chen, Lingna, Pei, Dong, Jin, Xiaobai, Zhang, Huijin
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier 01.03.2015
• Subjects: Biotechnological development; DNA marker; Genetic diversity detection; Biotechnological development; Genetic diversity detection; DNA marker
• ISSN: 2215-017X; 2215-017X
• DOI: 10.1016/j.btre.2014.11.003

Development of more sensitive nuclear DNA markers for identification of species, particularly closely allied taxa has been a challenging task that has attracted interest from scientists in fields of biotechnological development and genetic diversity detection. In this study, the sequence of the ubiquitin ligase gene ( ) region of nuclear DNA was tested for applicability and efficacy in revealing genetic diversity of walnut resources, with an emphasis on inter- and intra-specific levels. Analysis on genetic relationship among the taxa was conducted with the neighbor-joining (NJ) method. The number of variable bases in the region was 20 sites. All nine taxa (species/variety/cultivars) were distinguished using the sequence. In addition, each taxon was characterized molecularly with a unique nucleotide molecular formula using ten variable base sites derived from the nuclear DNA gene sequence. This study presents a good complementary methodology for developing new DNA markers for identification of genus .