Investigating immune and non‐immune cellular profiles in recurrent respiratory papillomatosis by multi‐omics

• Published in: Clinical and translational medicine Vol. 14; no. 3; pp. e1570 - n/a
• Main Authors: Niu, Zijie, Xiao, Yang, Li, Yiran, Zhou, Sihan, Liu, Meiyu, Li, Fangyuan, Zhang, Yaran, Wang, Jun, Wu, Xunyao
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.03.2024; Wiley
• Subjects: Angiogenesis; Cancer; Cells; Conflicts of interest; Genomics; Growth factors; Human papillomavirus; Humans; Hypoxia; Lymphocytes; Multiomics; Neutrophils; Papillomavirus Infections - genetics; Pediatrics; Respiratory Tract Infections - genetics; Tumors; Beijing China; China
• ISSN: 2001-1326; 2001-1326
• DOI: 10.1002/ctm2.1570

In this study, we performed multi-omics analysis for cell heterogeneity of tumour microenvironment (TME) and would provide a valuable source for developing new targets (Figure 1A). Phenotype analysis of DCs showed increased human leukocyte antigen (HLA)-DR+ and IDO1+ but decreased percentage of CD40+ DC in TILs compared with PBMCs (Figure 2E). Since high-risk HPV could trigger a regional Th2 inflammation at an incipient period and promote the gradual progression of cervical carcinoma,5,6 we inferred that the preferential accumulation of Th2, Treg, and immunosuppressive DCs in TILs might be an essential mechanism for tumour progression in RRP patients. Competitive gene set variation analysis (GSVA) was completed to unveil the biological positions of each cellular cluster in RRP tumourigenicity and advancement. [...]the enriched inflammatory pathways such as “IL6-JAK-STAT3 signalling,” “Hypoxia,” “metabolism-related pathways” as well as “inflammatory response” were observed in CXCL13+CXCL6+MMP13+ FLS but not MMP11+ FLS by GSVA analysis (Figure 3I).