Immobilization of UDP‐Galactose on an Amphiphilic Resin
Nucleotide sugars are activated sugar precursors used by glycosyltransferases to form glycans. Here we report the first examples of resin‐bound nucleotide sugars: fluorinated UDP‐galactose and UDP‐galactose. They were conjugated to a polyethylene glycol resin through the C6 position with an SMCC lin...
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Published in | European journal of organic chemistry Vol. 2018; no. 7; pp. 908 - 914 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
WEINHEIM
Wiley
21.02.2018
Wiley Subscription Services, Inc |
Subjects | |
Online Access | Get full text |
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Summary: | Nucleotide sugars are activated sugar precursors used by glycosyltransferases to form glycans. Here we report the first examples of resin‐bound nucleotide sugars: fluorinated UDP‐galactose and UDP‐galactose. They were conjugated to a polyethylene glycol resin through the C6 position with an SMCC linker in good to excellent yields. This synthesis represents an efficient method to access resin‐bound nucleotide sugars in a modular approach that enables modifications of the sugar or nucleotide before conjugation. The system has been conceived as a new chemistry‐based screening system for enzymes that use UDP‐galactose.
Here we report the first example of resin‐bound nucleotide sugars: fluorinated UDP‐Galactose and UDP‐Galactose. This synthesis represents an efficient method to access resin‐bound nucleotide sugars in a modular approach that enables modifications of the sugar or nucleotide before conjugation. |
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Bibliography: | FCT |
ISSN: | 1434-193X 1099-0690 |
DOI: | 10.1002/ejoc.201701620 |