Short-term studies of ovarian metabolism in the ewe

• Published in: Animal reproduction science Vol. 47; no. 1; pp. 43 - 58
• Main Authors: Rabiee, A.R., Lean, I.J., Gooden, J.M., Miller, B.G.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.05.1997
• Subjects: ABSORCION DE SUSTANCIAS NUTRITIVAS; ABSORPTION DE SUBSTANCES NUTRITIVES; Acetates - metabolism; Analysis of Variance; Animals; BREBIS; CHOLESTEROL; Cholesterol - metabolism; CICLO ESTRAL; COLESTEROL; CYCLE OESTRAL; Estradiol - blood; Estrus - metabolism; Estrus - physiology; EWES; Fatty Acids, Nonesterified - metabolism; Fatty Acids, Nonesterified - pharmacokinetics; Female; FOLICULOS OVARICOS; FOLLICULE OVARIEN; GLUCOSA; GLUCOSE; Glucose - metabolism; Glucose - pharmacokinetics; Gonadotropin-Releasing Hormone - pharmacology; Hydroxybutyrates - metabolism; LACTATE; LACTATES; Lactates - metabolism; LACTATOS; Luteinizing Hormone - blood; METABOLISM; METABOLISME; METABOLISMO; METABOLITE; METABOLITES; METABOLITOS; NUTRIENT UPTAKE; OESTROUS CYCLE; OVARIAN FOLLICLES; Ovary; Ovary - metabolism; OVEJA; PROGESTERONA; PROGESTERONE; Progesterone - blood; Radioimmunoassay - methods; Radioimmunoassay - veterinary; Reproduction - physiology; Sheep - blood; Sheep - metabolism; Sheep - physiology; Sheep-ovary; Time Factors; Ovary; Progesterone; Metabolism; Sheep-ovary; Oestrous cycle
• ISSN: 0378-4320; 1873-2232
• DOI: 10.1016/S0378-4320(96)01623-5

The ovarian uptake of metabolites in anaesthetised ewes was determined. In both studies, catheters were inserted into the ovarian vein and femoral artery, and Transonic flow transducers were placed around the ovarian arterio-venous plexus. Arterio-venous differences in glucose, lactate, free fatty acids (FFA), 3-hydroxybutyrate (3-OHB), acetate, cholesterol and progesterone and oestradiol-17β levels were determined every 10 min over a 3.5 h period. In study one, glucose uptake was significant in three sheep, and one sheep only had a significant uptake of FFA. Ovarian 3-OHB uptake was significant in two sheep. Significant uptake of acetate or cholesterol was identified in one sheep. Progesterone secretion was significant in three sheep and two sheep had significant progesterone uptake. In study 2, glucose uptake was significant in four sheep and lactate release was significant in the same sheep. There was uptake of FFA and 3-OHB, cholesterol, and acetate in each of three different sheep. Oestradiol-17β output was significant for sheep in oestrus and prooestrus. While the effects of gonadotrophin-releasing hormone (GnRH) treatment were confounded by time spent under anaesthesia, exogenous GnRH appeared to have no significant effect on the uptake of most metabolites and steroid hormone outputs. The metabolic requirements for energy and precursors for progesterone was small. Glucose was the major source of energy for the ovary and appears to be metabolised through anaerobic pathways, as indicated by significant lactate output.