Fabricating a Kidney Cortex Extracellular Matrix-Derived Hydrogel
Extracellular matrix (ECM) provides important biophysical and biochemical cues to maintain tissue homeostasis. Current synthetic hydrogels offer robust mechanical support for in vitro cell culture but lack the necessary protein and ligand composition to elicit physiological behavior from cells. This...
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Published in | Journal of visualized experiments no. 140 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
MyJove Corporation
13.10.2018
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Subjects | |
Online Access | Get full text |
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Summary: | Extracellular matrix (ECM) provides important biophysical and biochemical cues to maintain tissue homeostasis. Current synthetic hydrogels offer robust mechanical support for in vitro cell culture but lack the necessary protein and ligand composition to elicit physiological behavior from cells. This manuscript describes a fabrication method for a kidney cortex ECM-derived hydrogel with proper mechanical robustness and supportive biochemical composition. The hydrogel is fabricated by mechanically homogenizing and solubilizing decellularized human kidney cortex ECM. The matrix preserves native kidney cortex ECM protein ratios while also enabling gelation to physiological mechanical stiffnesses. The hydrogel serves as a substrate upon which kidney cortex-derived cells can be maintained under physiological conditions. Furthermore, the hydrogel composition can be manipulated to model a diseased environment which enables the future study of kidney diseases. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Undefined-2 Correspondence to: Jonathan Himmelfarb at himmej@uw.edu, Ying Zheng at yingzy@uw.edu |
ISSN: | 1940-087X 1940-087X |
DOI: | 10.3791/58314 |