Benzimidazole and imidazole fungicide analysis in grape and wine samples using a competitive enzyme-linked immunosorbent assay

• Published in: Analytical methods Vol. 7; no. 21; pp. 9158 - 9165
• Main Authors: Uclés, Ana, García, Antonio Valverde, Gil García, María Dolores, Aguilera del Real, Ana María, Fernández-Alba, Amadeo R
• Format: Journal Article
• Language: English
• Published: 01.01.2015
• Subjects: Assaying; ELISA; Fungicides; Grapes; Liquid chromatography; Pesticides; Residues; Vitaceae; Wines
• ISSN: 1759-9660; 1759-9679
• DOI: 10.1039/c5ay01048a

This manuscript reports a quantitative pesticide residue method analysis employing an enzyme-linked immunosorbent assay (ELISA) for detecting carbendazim, imazalil and thiabendazole pesticide residues in vegetable samples. The results have been compared with those obtained from a liquid chromatography system coupled to a triple quadrupole mass spectrometer (LC-ESI-QqQ-MS). A validation study was undertaken to establish this method's performance characteristics on the 3 pesticide residues in two commodities (table grapes and wine). The carbendazim, imazalil and thiabendazole ELISA showed an IC 50 reporting limit ranging from 0.5 to 13.8 µg kg −1 . The limit of quantification of the developed assay was 5 µg kg −1 for both grape and wine matrices. The optimised assay was compared with a reference procedure based on liquid chromatography using samples acquired from various supermarkets, and a good correlation between both methods was found. Standard curves of the carbendazim, imazalil and thiabendazole immunoassays in a white wine matrix and the IC 50 values for the three immunoassays.