The lignin synthesis related genes and lodging resistance of Fagopyrum esculentum

• Published in: Biologia plantarum Vol. 61; no. 1; pp. 138 - 146
• Main Authors: Hu, D., Liu, X. B., She, H. Z., Gao, Z., Ruan, R. W., Wu, D. Q., Yi, Z. L.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2017; Springer Nature B.V; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Alcohol dehydrogenase; Alcohols; Ammonia; Biomedical and Life Sciences; Biosynthesis; Buckwheat; Caffeic acid; Caffeic acid O-methyltransferase; Caffeoyl-CoA O-methyltransferase; Cinnamate 4-hydroxylase; Cinnamoyl-CoA reductase; Cinnamyl-alcohol dehydrogenase; common buckwheat; Cultivars; enzyme activities; Enzymes; Gas chromatography; Gene expression; Genes; Life Sciences; Lignin; Mass spectrometry; Mass spectroscopy; Maturation; Methyltransferase; Monomers; Original Paper; Peroxidase; Phenylalanine; Plant Sciences; Polymerase chain reaction; Synthesis; common buckwheat; enzyme activities; gene expression
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-016-0685-4

Lignin is closely related to the lodging resistance of common buckwheat ( Fagopyrum esculentum Moench.). However, the characteristics of lignin synthesis related genes have not yet been reported. We investigated the lignin biosynthesis gene expression, activities of related enzymes, and accumulation of lignin monomers during branching stage, bloom stage, and milky ripe stage by real-time quantitative PCR, UVspectrophotometry, and gas chromatography-mass spectrometry in the 2 nd internode of three common buckwheat cultivars with different lodging resistance. The results showed that lignin content and the activity of phenylalanine ammonia lyase (PAL), 4-coumarate: CoA ligase (4CL), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD) were closely related to the lodging resistance of common buckwheat. Further, we studied gene expression of cinnamate 4-hydroxylase (C4H), caffeoyl-CoA O-methyltransferase (CCoAOMT), ferulate 5-hydroxylase (F5H), cinnamoyl-CoA reductase (CCR), and caffeic acid O-methyltransferase (COMT). The lignin biosynthesis genes were divided into three classes according to their expression pattern: 1 ) expression firstly increasing and then descending ( PAL , 4CL , CAD , C4H , CCoAOMT , F5H , and CCR ), 2 ) expression remaining constant during maturation ( C3H ), and 3 ) expression decreasing with maturation ( COMT ). The present study provides preliminary insights into the expression of lignin biosynthesis genes in common buckwheat, laying a foundation for further understanding the lignin biosynthesis.