Pregnancy rates relative to recipient plasma progesterone levels on the day of nonsurgical transfer of frozen/thawed bovine embryos

• Published in: Theriogenology Vol. 23; no. 4; pp. 631 - 639
• Main Authors: Niemann, H., Sacher, B., Elsaesser, F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1985
• Subjects: corpus luteum quality; estrus interval; frozen/thawed bovine embryos; plasma progesterone levels; pregnancy rates; plasma progesterone levels; pregnancy rates; corpus luteum quality; frozen/thawed bovine embryos; estrus interval
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/0093-691X(85)90197-9

A total of 71 synchronized dairy heifers (Holstein Friesian × German Black Pied) were used as recipients of seven-day old frozen/thawed bovine embryos. Plasma progesterone concentrations and corpus luteum quality on the day of nonsurgical transfer (= day 7) were determined and related to pregnancy rates or estrus intervals in nonpregnant recipients. A total of 32 recipients (45.1 %) maintained pregnancy; 39 recipients (54.9 %) did not. No significant differences could be detected between progesterone levels in recipients that remained pregnant (3.14 ± 0.24 ng/ml; x ± SEM ) and those that did not maintain pregnancy (3.23 ± 0.28 ng/ml). Optimal progesterone levels were between 2 and 5 ng/ml coinciding with a pregnancy rate of 51.1 % ( 24 47 ). Pregnancy rates apparently were decreased when progesterone levels were below 2 ng/ml (35.3 %; 6 17 ) or above 5 ng/ml (28.6 %; 2 7 ). Hence, optimal progesterone levels were identical to those for freshly collected embryos reported previously by Remsen et al. (1). Bovine corpus luteum quality graded by rectal palpation was related to some extent to progesterone levels but not to pregnancy rates. Out of 39 nonpregnant recipients seven animals (17.9 %) with a mean plasma progesterone level of 3.76 ± 0.72 ng/ml showed an extended estrus interval of more than 55 days, probably indicating early embryonic mortality. Progesterone levels did not significantly differ between nonpregnant recipients with estrus intervals of various length. Plasma progesterone levels at the time of transfer are of limited diagnostic value for screening recipients prior to transfer of frozen/thawed embryos.