Reconstitution of Nucleosomes with Differentially Isotope-labeled Sister Histones

• Published in: Journal of visualized experiments no. 121
• Main Author: Liokatis, Stamatios
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 26.03.2017
• Subjects: Biochemistry; Gene Expression Regulation - physiology; Histones - chemistry; Isotope Labeling; Magnetic Resonance Spectroscopy; Nucleosomes; Protein Processing, Post-Translational
• ISSN: 1940-087X; 1940-087X
• DOI: 10.3791/55349

Asymmetrically modified nucleosomes contain the two copies of a histone (sister histones) decorated with distinct sets of Post-translational Modifications (PTMs). They are newly identified species with unknown means of establishment and functional implications. Current analytical methods are inadequate to detect the copy-specific occurrence of PTMs on the nucleosomal sister histones. This protocol presents a biochemical method for the in vitro reconstitution of nucleosomes containing differentially isotope-labeled sister histones. The generated complex can be also asymmetrically modified, after including a premodified histone pool during refolding of histone subcomplexes. These asymmetric nucleosome preparations can be readily reacted with histone-modifying enzymes to study modification cross-talk mechanisms imposed by the asymmetrically pre-incorporated PTM using nuclear magnetic resonance (NMR) spectroscopy. Particularly, the modification reactions in real-time can be mapped independently on the two sister histones by performing different types of NMR correlation experiments, tailored for the respective isotope type. This methodology provides the means to study crosstalk mechanisms that contribute to the formation and propagation of asymmetric PTM patterns on nucleosomal complexes.