FITC delivery into plant cells using magnetic single-walled carbon nanotubes

In this paper, fluorescein isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forc...

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Published inJournal of nanoscience and nanotechnology Vol. 12; no. 8; p. 6287
Main Authors Hao, Yuzhi, Yang, Xiaoyan, Shi, Yongzhong, Xing, James, Marowitch, Janet, Chen, Jianmin, Chen, Jie
Format Journal Article
LanguageEnglish
Published United States 01.08.2012
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Summary:In this paper, fluorescein isothiocyanate (FITC) was covalently bonded with magnetic single-walled carbon nanotubes (mSWCNTs) that were purified using our previous method. To demonstrate our design, mSWCNT-FITC was delivered into plant cells (canola and carrot cells) driven by external magnetic forces. From FACS results, the FITC delivery efficiency was about 100% for both two canola and carrot protoplasts, which were further confirmed by the confocal and sectional TEM images. Some mSWCNTs were found trapped both inside the endosomes of canola protoplast and outside endosome near the nuclear membrane of carrot protoplast according to the sectional TEM images. All results showed that mSWCNT is a good delivery carrier for biomolecules.
ISSN:1533-4880
DOI:10.1166/jnn.2012.6449