Enhancing T cell responses and tumour immunity by vaccination with peptides conjugated to a weak NKT cell agonist
Activated NKT cells can stimulate antigen-presenting cells leading to enhanced peptide antigen-specific immunity. However, administration of potent NKT cell agonists like alpha-galactosylceramide (alpha-GalCer) can be associated with release of high levels of cytokines, and in some situations, hepat...
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Published in | Organic & biomolecular chemistry Vol. 17; no. 5; pp. 1225 - 1237 |
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Main Authors | , , , , , , , , , , , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
CAMBRIDGE
Royal Soc Chemistry
31.01.2019
Royal Society of Chemistry |
Subjects | |
Online Access | Get full text |
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Summary: | Activated NKT cells can stimulate antigen-presenting cells leading to enhanced peptide antigen-specific immunity. However, administration of potent NKT cell agonists like alpha-galactosylceramide (alpha-GalCer) can be associated with release of high levels of cytokines, and in some situations, hepatotoxicity. Here we show that it is possible to provoke sufficient NKT cell activity to stimulate strong antigen-specific T cell responses without these unwanted effects. This was achieved by chemically conjugating antigenic peptides to alpha-galactosylphytosphingosine (alpha-GalPhs), an NKT cell agonist with very weak activity based on structural characterisation and biological assays. Conjugation improved delivery to antigen-presenting cells in vivo, while use of a cathepsin-sensitive linker to release the alpha-GalPhs and peptide within the same cell promoted strong T cell activation and therapeutic anti-tumour responses in mice. The conjugates activated human NKT cells and enhanced human T cell responses to a viral peptide in vitro. Accordingly, we have demonstrated a means to safely exploit the immunostimulatory properties of NKT cells to enhance T cell activation for virus-and tumour-specific immunity. |
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ISSN: | 1477-0520 1477-0539 |
DOI: | 10.1039/c8ob02982b |