Comparative metabolism of aschantin in human and animal hepatocytes

• Published in: Archives of pharmacal research Vol. 47; no. 2; pp. 111 - 126
• Main Authors: Lee, Min Seo, Shim, Hyun Joo, Cho, Yong-Yeon, Lee, Joo Young, Kang, Han Chang, Song, Im-Sook, Lee, Hye Suk
• Format: Journal Article
• Language: English
• Published: Seoul Pharmaceutical Society of Korea 01.02.2024; 대한약학회
• Subjects: Animals; Benzodioxoles; Catechols; Cytochrome P-450 CYP2C19 - metabolism; Cytochrome P-450 CYP2C9 - metabolism; Cytochrome P-450 CYP3A - metabolism; Cytochrome P-450 Enzyme System - metabolism; Dogs; Hepatocytes - metabolism; Humans; Kinetics; Lignans; Medicine; Mice; Microsomes, Liver - metabolism; Pharmacology/Toxicology; Pharmacy; Rats; Research Article; 약학; GSH adduct; Metabolite identification; 1,3-benzodioxole; Cytochrome P450; Aschantin
• ISSN: 0253-6269; 1976-3786
• DOI: 10.1007/s12272-023-01483-w

Aschantin, a tetrahydrofurofuran lignan with a 1,3-benzodioxole group derived from Flos Magnoliae , exhibits antioxidant, anti-inflammatory, cytotoxic, and antimicrobial activities. This study compared the metabolic profiles of aschantin in human, dog, mouse, and rat hepatocytes using liquid chromatography–high-resolution mass spectrometry. The hepatic extraction ratio of aschantin among the four species was 0.46–0.77, suggesting that it undergoes a moderate-to-extensive degree of hepatic metabolism. Hepatocyte incubation of aschantin produced 4 phase 1 metabolites, including aschantin catechol (M1), O -desmethylaschantin (M2 and M3), and hydroxyaschantin (M4), and 14 phase 2 metabolites, including O -methyl-M1 (M5 and M6) via catechol O -methyltransferase (COMT), six glucuronides of M1, M2, M3, M5, and M6, and six sulfates of M1, M2, M3, M5, and M6. Enzyme kinetic studies using aschantin revealed that the production of M1, a major metabolite, via O -demethylenation is catalyzed by cytochrome 2C8 (CYP2C8), CYP2C9, CYP2C19, CYP3A4, and CYP3A5 enzymes; the formation of M2 ( O -desmethylaschantin) is catalyzed by CYP2C9 and CYP2C19; and the formation of M4 is catalyzed by CYP3A4 enzyme. Two glutathione (GSH) conjugates of M1 were identified after incubation of aschantin with human and animal liver microsomes in the presence of nicotinamide adenine dinucleotide phosphate and GSH, but they were not detected in the hepatocytes of all species. In conclusion, aschantin is extensively metabolized, producing 18 metabolites in human and animal hepatocytes catalyzed by CYP, COMT, UDP-glucuronosyltransferase, and sulfotransferase. These results can help in clarifying the involvement of metabolizing enzymes in the pharmacokinetics and drug interactions of aschantin and in elucidating GSH conjugation associated with the reactive intermediate formed from M1 (aschantin catechol).