Structure elucidation and immunological activity of a novel pectic polysaccharide from the stems of Avicennia marina

• Published in: European food research & technology Vol. 236; no. 2; pp. 243 - 248
• Main Authors: Fang, Xubo, Chen, Xiaoe
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.02.2013; Springer Nature B.V
• Subjects: Acids; Agriculture; Analytical Chemistry; Arabinose; Avicennia marina; B-lymphocytes; Biomedical research; Biotechnology; Chemistry; Chemistry and Materials Science; Disaccharides; Exploration; Flowers & plants; Food; Food Science; Forestry; Immunology; lymphocyte proliferation; Lymphocytes; Lymphocytes B; Lymphocytes T; Molecular weight; Original Paper; Physiology; Polysaccharides; rhamnogalacturonan; Sodium; Studies; T-lymphocytes; China; Rhamnogalacturonan; Polysaccharides; Pectin
• ISSN: 1438-2377; 1438-2385
• DOI: 10.1007/s00217-012-1877-6

The structure of HAM-3-IIb-II, an acidic pectic polysaccharide in Avicennia marina , was characterized in detail and the immunological activity was investigated in this study. The results showed that HAM-3-IIb-II contained Rha, Ara, Gal, Glc, and GalA in a molar ratio of 1:2.8:2.4:6.1 and that HAM-3-IIb-II is a type I rhamnogalacturonan with branches of arabinose chains, galactosyl chains, and arabinogalactosyl chains. The backbone of HAM-3-IIb-II is mainly composed of disaccharide repeat units, →4)-α- d -GalA-(1 → 2)-α- l -Rha p -(1→. These repeats are predominantly linked to the O-4 of 1,2,4-linked α- l -Rha p in the branches of arabinose chains, galactosyl chains, and arabinogalactosyl chains. HAM-3-IIb-II had a significant effect on lipopolysaccharide-induced B lymphocyte proliferation, but it had little effect on concanavalin A-induced T lymphocyte proliferation compared with the control. This study provides a foundation for the exploration and utilization of A. marina in the future.