The Production of Pluripotent Stem Cells from Mouse Amniotic Fluid Cells Using a Transposon System

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exh...

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Bibliographic Details
Published inJournal of visualized experiments no. 120
Main Authors Bertin, Enrica, Piccoli, Martina, Franzin, Chiara, Nagy, Andras, Mileikovsky, Maria, De Coppi, Paolo, Pozzobon, Michela
Format Journal Article
LanguageEnglish
Published United States MyJove Corporation 28.02.2017
Subjects
Amniotic Fluid - cytology
Animals
Cell Differentiation
Cell Line
Cellular Reprogramming
Developmental Biology
Female
Induced Pluripotent Stem Cells - cytology
Induced Pluripotent Stem Cells - metabolism
Mice
Pregnancy
Pregnancy, Animal
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Summary:Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Here, we produced iPS cells from mouse amniotic fluid cells, using a non-viral-based transposon system. All obtained iPS cell lines exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo. This strategy opens up the possibility of using cells from diseased fetuses to develop new therapies for birth defects.
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Correspondence to: Michela Pozzobon at m.pozzobon@irpcds.org
ISSN:1940-087X
1940-087X
DOI:10.3791/54598